- 询价
- Miltenyi Biotec
- 德国
- 130-129-454
- 2026年01月21日
- Flow cytometry
- mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Melanoma (MCSP)
- 亚型：
mouse IgG1κ
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
EP-1
- 标记物：
PE
- 适应物种：
human
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
mouse
- 应用范围：
Flow cytometry
- 浓度：
1:50
- 靶点：
Melanoma (MCSP)
- 抗体英文名：
Melanoma (MCSP) Antibody, anti-human
- 抗体名：
Melanoma (MCSP) Antibody, anti-human
- 规格：
30 tests in 60 µL
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
Western Blotting with Horseradish Pe
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS
Phycoerythrin conjugation protocol
30 µl DTT (0.5M, i.e. 77 mg/ml in PBS). Incubate for 30 mins at room temperature. Separate derivatized PE and Ab from activators on G-10 columns. You should now have approximately 2 ml of each reactant. Mix derivatized
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