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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Cytochrome c
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,2-8℃
- 克隆性:
REA702
- 标记物:
FITC
- 适应物种:
human, mouse, rat
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
human cell line
- 应用范围:
Intracellular flow cytometry
- 浓度:
1:50
- 靶点:
Cytochrome c
- 抗体英文名:
Cytochrome c Antibody, anti-human/mouse/rat, REAfinity™
- 抗体名:
Cytochrome c Antibody, anti-human/mouse/rat, REAfinity™
- 规格:
100 tests in 200 µL
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文献和实验secondary antibody review -- data from 99 publications
epithelial Caco2/bbe cells Bio-Rad 10 rhodamine immunocytochemistry 1:100 detect phospho-CREB in human colonic epithelial Caco2/bbe cells Bio-Rad 11 goat IgG FITC immunohistochemistry 1:40 detect ameloblastin in mouse postnatal mandibular molars
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
兔细胞色素C(Cytochrome C)ELISA试剂盒 说明书
Cytochrome C 的水平可能有较大差异,请根据实际情况灵活掌握稀释度) 5. 细胞培养上清或组织匀浆 10 倍稀释 (410ul 的标本稀释液+ 50ul 样本+ 20ul 的 1N HCI+20ul 的 1N NaOH) 。 检测程序 1. 加样:每孔各加入标准品或待测样品(已激活) 100ul ,将反应板充分混匀后置 37 ℃ 120 分钟。 2. 洗板:用洗涤液将反应板充分洗涤 4-6 次,向滤纸上印干。 3.
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