- 询价
- Miltenyi Biotec
- 德国
- 130-099-318
- 2025年12月09日
- Intracellular flow cytometry
- human cell line
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
c-Cbl pY774
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
REA118
- 标记物：
PerCP-Vio 700
- 适应物种：
human
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Intracellular flow cytometry
- 浓度：
1:11
- 靶点：
c-Cbl pY774
- 抗体英文名：
c-Cbl pY774 Antibody, anti-human, REAfinity™
- 抗体名：
c-Cbl pY774 Antibody, anti-human, REAfinity™
- 规格：
30 tests in 300 µL
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
of 2 N HCl x30'. Wash 3x in PBS. Block in 1 mL 10% Horse serum, x30' at room temp. Wash 1x in PBS. Add 0.3 mL Biotinylated anti-BrdU (1:300, with 1% horse serum in PBS). Incubate at 4ºC o.n. or 2 hr. at room temp. Wash 3x in PBS.
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
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