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Bcl-2 Antibody, anti-mouse, AP

C, REAfinity™, 30 µg in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-123-567
  • 2025年12月09日
  • Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • mouse
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Bcl-2

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA356

    • 标记物

      APC

    • 适应物种

      mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:50

    • 靶点

      Bcl-2

    • 抗体英文名

      Bcl-2 Antibody, anti-mouse, REAfinity™

    • 抗体名

      Bcl-2 Antibody, anti-mouse, REAfinity™

    • 规格

      30 µg in 200 µL

    Identification and enumeration of Bcl-2+ cells by flow cytometryClone REA356 recognizes the mouse Bcl-2 antigen, a 26 kDa intracellular single-pass membrane protein found primarily in the mitochondrion outer membrane, the nucleus membrane, and the endoplasmic reticulum membrane. Bcl-2 is the founding member of the Bcl-2 family of regulator proteins and it forms homodimers or heterodimers with other Bcl-2 family members. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Bcl-2 regulates cell death by controlling the mitochondrial membrane permeability and it appears to function in a feedback loop system with caspases. Caspase activity is either inhibited by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. | Additional information: Clone REA356 displays negligible binding to Fc receptors.

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    图标文献和实验
    相关实验
    • APC和TSC-2 cDNA的长5'RACE

      30s,接着40个循环的94℃ 15 s,65℃ 20 s,68℃ 5 min],其中使用了1单位的ELONGASE酶混合物[PCR 50ml终体积,其中60 mM Tris-SO4(pH9.1),18 mM (NH4)2SO4,2 mM MgSO4,dNTP每个200 µM,引物每个400 nM ],其中分别是AAP和引物TSC-2-2826(A带)或引物TSC-2-1732(B带)一起使用。每个PCR产物(带1和带3)取10µl 在0.8%琼脂糖上,1倍TAE凝胶电泳和SYBRò绿染色(分子

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

      of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G

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