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CD21/CD35 Antibody, anti-mouse

, PE, 30 µg in 1 mL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-102-440
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • rat
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD21/CD35

    • 亚型

      rat IgG2aκ

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      7E9 

    • 标记物

      PE

    • 适应物种

      mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      rat

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:10

    • 靶点

      CD21/CD35

    • 抗体英文名

      CD21/CD35 Antibody, anti-mouse

    • 抗体名

      CD21/CD35 Antibody, anti-mouse

    • 规格

      30 µg in 1 mL

    Detection of mouse CD21+ and CD35+ cellsClone 7E9 reacts with an epitope shared by mouse CD21 and CD35, also known as CR2 and CR1. CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which produce cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed on the majority of peripheral B lymphocytes, on the majority of resident peritoneal macrophages, on mast cells, activated granulocytes, and follicular dendritic cells. CD21 forms part of a signal transduction complex with CD19 and CD81, which are associated with the antigen receptor on B lymphocytes.

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    图标文献和实验
    相关实验
    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC™ Anti-Mouse Bead Kit 1)        Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • T-cell dependent antigen specific in situ staining (Pe)

      6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block

    • Western Blotting with Horseradish Pe

      . 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL

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