- 询价
- Miltenyi Biotec
- 德国
- 130-095-749
- 2025年12月09日
- Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Functional assay
- human
- human
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- 详细信息
- 技术资料
- 免疫原：
TNF-α
- 亚型：
human IgG1
- 形态：
Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
- 保存条件：
避光，2-8℃
- 克隆性：
cA2
- 标记物：
pure-functional grade
- 适应物种：
human
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human
- 应用范围：
Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Functional assay
- 靶点：
TNF-α
- 抗体英文名：
TNF-α Antibody, anti-human
- 抗体名：
TNF-α Antibody, anti-human
- 规格：
500 µg in 0.5 mL
Unconjugated antibody for functional assays, e.g., neutralization of TNF-α activityTumor necrosis factor alpha (TNF-α) is produced by cells that are involved in inflammatory immune responses. TNF-α is secreted by activated CD4+ T cells, monocytes, macrophages, NK cells, and neutrophils. | Anti-TNF-α antibodies have been designed for intracellular staining of TNF-α–producing cells. Cells can be stimulated for TNF-α production, for example, by polyclonal stimulation with mitogens. For induction of TNF-α production by antigen-specific T cells, cells are restimulated with the respective antigen. TNF-α can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, TNF-α–producing cells can be stained intracellularly with Anti-TNF-α antibodies. Staining for surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the TNF-α–producing cells.
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TNF-α Antibody, anti-human, pure-functional grade, 500 µg in 0.5 mL
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