- 询价
- Miltenyi Biotec
- 德国
- 130-121-049
- 2026年04月20日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Functional assay
- mouse
- human, non-human primate
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD28
- 亚型：
mouse IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
15E8
- 标记物：
APC-Vio 770
- 适应物种：
human, non-human primate
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
mouse
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Functional assay
- 浓度：
1:50
- 靶点：
CD28
- 抗体英文名：
CD28 Antibody, anti-human
- 抗体名：
CD28 Antibody, anti-human
- 规格：
100 tests in 200 µL
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文献和实验Use a 1L bag for CD3 T cell separation. Add 100 ml of air to the bag. 3) Add 5 x 108 CD3 T cells to the 1L bag in 50-60 ml Buffer 1. 4) Add 4.0 ml (corresponding to 1.6 x 109 Dynabeads®) of washed Dynabeads® CD3⁄CD28
Dual- and Triple-Color Fluorospot
stimulatory agents of choice and anti-CD28 (0.1 μg/ml; seeNote 4), in a final volume of 100–150 μl/well. The number of cells per well is dependent on the stimulating agent used (seeNote 5). b. Incubate the plate overnight at 37°C in a humidified
ELISA Procedure for Measuring Serum Antibody Titer
Dilution ScheduleDilution ofAntiserum Volume of 1% BSA/PBS-T (µL) Volume of PreviousDilution (µL) 1:100 1000 1000 of 1:50 1:200 1000 1000 of 1:100 1:400 1000 1000 of 1:200 1:800 1000 1000 of 1:400 ... ... ... 1:102,400 1000 1000 of 1:51,200 11. Vortex
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