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- 湖北省武汉市
- Schizosaccharomyces pombe lig4 重组蛋白表达
- 2025年12月08日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：O74833
基因名：lig4
蛋白名：DNA ligase 4
蛋白别名：
DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
O74833相关研究文献：
1. The genome sequence of Schizosaccharomyces pombe.
2. Comparative functional genomics of the fission yeasts.
3. Protection of telomeres by the Ku protein in fission yeast.
4. The fission yeast Taz1 protein protects chromosomes from Ku-dependent end-to-end fusions.
5. Histone H2A phosphorylation controls Crb2 recruitment at DNA breaks, maintains checkpoint arrest, and influences DNA repair in fission yeast.
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文献和实验Electroporation of Schizosaccharomyces pombe
Many of the techniques that have been developed for the manipulation of the budding yeast Saccharomyces cerevisiae have now been adapted to be used on the alternative host, Schizosaccharomyces pombe . One particularly important technique
Genotoxicity Testing in Schizosaccharomyces pombe
During the last 20 yr, the established molecular relationship between mutagenicity and carcinogenicity has been exploited in the development of numerous microbial tests designed to detect mutagenic activity and hence predict carcinogenic
In Vitro Excision Repair Assay in Schizosaccharomyces pombe
The measurement of DNA excision repair activity in vitro, originally developed by Wood and co-workers (1 ), utilizes transcriptionally active protein extracts obtained from mammalian cells by the method of Manley et al. (2 ) (see Chapter 29
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