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NANOG Antibody抗体,orb1239398,bi

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  • ¥3770
  • biorbyt已认证
  • orb1239398
  • 英国
  • 2025年12月08日
  • ELISA, ICC, IF, IHC-P, WB
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      NANOG Antibody抗体

    • 抗体英文名

      NANOG Antibody

    • 靶点

      NANOG

    • 浓度

      1 mg/mL

    • 应用范围

      ELISA, ICC, IF, IHC-P, WB

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb1239398

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG.The immunogen is located within amino acids 130 - 180 of NANOG.

    • 规格

      0.02 mg

    产品描述:NANOG Antibody

    别名:NANOG Antibody: Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

    免疫原:NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG.The 免疫原 is located within amino acids 130 - 180 of NANOG.

    预测反应性:Bovine, Gallus

    分子量:Predicted: 35 kD Observed: 35 kD

    应用注释:WB: 1-2 μg/mL; ICC/IF: 20 μg/mL; IHC: 1-5 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.

    防腐剂:NANOG Antibody is supplied in PBS containing 0.02% sodium azide.

    纯化:NANOG Antibody is affinity chromatography purified via peptide column.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    NCBI:EAW88651

    UniProt ID:Q9H9S0

    Note:For research use only.

    产品细节图片1
    Immunoflouorescence Validation of NANOG in HeLa Cells. Immunofluorescent analysis of PFA-fixed HeLa cells labeling NANOG with orb1239398 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

    产品细节图片2
    WB Validation in HeLa Cells. Loading: 15 µg of lysate. Antibodies: NANOG orb1239398, 2 µg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

    产品细节图片3
    Western Blot Validation in Mouse and Rat Testis. Loading: 15 µg of lysates per lane. Antibodies: NANOG orb1239398, 2 µg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

    产品细节图片4
    Immunohistochemistry Validation of NANOG in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-NANOG antibody (orb1239398) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    产品细节图片5
    Immunohistochemistry Validation of NANOG in Mouse Testis. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NANOG antibody (orb1239398) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    产品细节图片6
    Immunohistochemistry Validation of NANOG in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NANOG antibody (orb1239398) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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    图标文献和实验
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    • Cell Metabolism:刘光慧 / 曲静评述长寿通路研究新发现

      数据集,作者发现昼夜节律调节器 CLOCK 对参与能量代谢的 Neg-MLS 基因的启动子区域显示出显着更高的结合亲和力,而 Pos-MLS 基因是 OCT4、NANOG、SOX2 和 KLF4 等多能性调节因子的调控靶点基因。 图:长寿的转录调控 综上所述,近期的研究确定了在具有 MLS 差异的不同物种中进化的转录组学特征及其调控网络,对生物学通路和调控网络的深入解析将增加我们对衰老及衰老相关疾病的理解,为干预措施的发展与完善提供了新的曙光。

    • 听说大家都准备做 m6A 了,你想好要怎么做了吗?

      5-mediated m6A-demethylation of NANOG mRNA。由标题便可以初步判断这个研究课题的套路:l 老瓶装新酒:缺氧微环境促进肿瘤细胞干性;l 选择了m6A的去甲基化执行者ALKBH5作为研究对象l 选择某一基因(NANOG)作为ALKBH5的靶标研究背景有两个:①已有NANOG在METTL3的调控下发生甲基化的报道,且NANOG作为一个多能干性因子,可促进乳腺癌干细胞(BCSC)的产生;②HIF介导了缺氧微环境对ALKBH5的促进作用。在此基础上,这个文章做了哪些研究?四

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