NANOG Antibody抗体,orb1239398,biorbyt

NANOG Antibody抗体,orb1239398,bi

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  • ¥3770
  • biorbyt已认证
  • orb1239398
  • 英国
  • 2025年12月08日
  • ELISA, ICC, IF, IHC-P, WB
  • Human, Mouse, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      NANOG Antibody抗体

    • 抗体英文名

      NANOG Antibody

    • 靶点

      NANOG

    • 浓度

      1 mg/mL

    • 应用范围

      ELISA, ICC, IF, IHC-P, WB

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb1239398

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG.The immunogen is located within amino acids 130 - 180 of NANOG.

    • 规格

      0.02 mg

    产品描述:NANOG Antibody

    别名:NANOG Antibody: Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

    免疫原:NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG.The 免疫原 is located within amino acids 130 - 180 of NANOG.

    预测反应性:Bovine, Gallus

    分子量:Predicted: 35 kD Observed: 35 kD

    应用注释:WB: 1-2 μg/mL; ICC/IF: 20 μg/mL; IHC: 1-5 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.

    防腐剂:NANOG Antibody is supplied in PBS containing 0.02% sodium azide.

    纯化:NANOG Antibody is affinity chromatography purified via peptide column.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    NCBI:EAW88651

    UniProt ID:Q9H9S0

    Note:For research use only.

    NANOG Antibody抗体,orb1239398,bi
    Immunoflouorescence Validation of NANOG in HeLa Cells. Immunofluorescent analysis of PFA-fixed HeLa cells labeling NANOG with orb1239398 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

    NANOG Antibody抗体,orb1239398,bi
    WB Validation in HeLa Cells. Loading: 15 µg of lysate. Antibodies: NANOG orb1239398, 2 µg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

    NANOG Antibody抗体,orb1239398,bi
    Western Blot Validation in Mouse and Rat Testis. Loading: 15 µg of lysates per lane. Antibodies: NANOG orb1239398, 2 µg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

    NANOG Antibody抗体,orb1239398,bi
    Immunohistochemistry Validation of NANOG in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-NANOG antibody (orb1239398) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    NANOG Antibody抗体,orb1239398,bi
    Immunohistochemistry Validation of NANOG in Mouse Testis. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NANOG antibody (orb1239398) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    NANOG Antibody抗体,orb1239398,bi
    Immunohistochemistry Validation of NANOG in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NANOG antibody (orb1239398) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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