- ￥1960
- BOSTER
- M00548-2-100ul
- 中国
- 2025年07月12日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | M00548-2 |
|---|---|
| 产品名称 | Anti-ZEB1 Antibody (monoclonal, 8B12D7) |
| 基因名 | ZEB1 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 200KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human AREB6/ZEB1 (LLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQ). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | ZEB1 (Zinc Finger E Box-Binding Homeobox 1), also called TCF8, NIL2A or DELTA-EF1, is a protein that in humans is encoded by the ZEB1 gene. Fluorescence in situ hybridization localized the ZEB1 gene to chromosome 10p11.2. Krafchak et al. (2005) demonstrated a complex (core plus secondary) binding site for TCF8 in the promoter of the COL4A3 gene, mutant in Alport syndrome and which encodes collagen type IV alpha-3. They detected expression of TCF8 in cornea. Nishimura et al. (2006) found that delta-Ef1 was upregulated during differentiation in a mouse smooth muscle cell (SMC) line. |
| 研究类别 | 1. Krafchak, C. M., Pawar, H., Moroi, S. E., Sugar, A., Lichter, P. R., Mackey, D. A., Mian, S., Nairus, T., Elner, V., Schteingart, M. T., Downs, C. A., Kijek, T. G., and 9 others. Mutations in TCF8 cause posterior polymorphous corneal dystrophy and ectopic expression of COL4A3 by corneal endothelial cells. Am. J. Hum. Genet. 77: 694-708, 2005. 2. Nishimura, G., Manabe, I., Tsushima, K., Fujiu, K., Oishi, Y., Imai, Y., Maemura, K., Miyagishi, M., Higashi, Y., Kondoh, H., Nagai, R. Delta-EF1 mediates TGF-beta signaling in vascular smooth muscle cell differentiation. Dev. Cell 11: 93-104, 2006. |
| Uniprot ID | ZEB1: P37275 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- ZEB1 antibody (M00548-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U87 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse lung tissue lysates.
Use mouse anti- ZEB1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ZEB1 at approximately 200KD. The expected band size for ZEB1 is at 124KD.|Figure 2. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human glioblastoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human thyroid cance tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human breast cance tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human Lung adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 8. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human testicular germ cell tumors tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 9. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 10. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure11.IF analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human Glioma tissues. The tissue section were stained using the Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1133).|Figure 12. ICC analysis using anti- ZEB1 antibody (M00548-2). was detected in immersion fixed U87 cell. Cells were stained using the Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog#BA1133).[/list_product_images]
Lane 1: U87 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse lung tissue lysates.
Use mouse anti- ZEB1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ZEB1 at approximately 200KD. The expected band size for ZEB1 is at 124KD.|Figure 2. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human glioblastoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human thyroid cance tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human breast cance tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human Lung adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 8. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human testicular germ cell tumors tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 9. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 10. IHC analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure11.IF analysis using anti- ZEB1 antibody (M00548-2). detected in paraffin-embedded section of human Glioma tissues. The tissue section were stained using the Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1133).|Figure 12. ICC analysis using anti- ZEB1 antibody (M00548-2). was detected in immersion fixed U87 cell. Cells were stained using the Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog#BA1133).[/list_product_images]
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Anti-ZEB1 Antibody (monoclonal, 8B12D7)(M00548-2-100ul)
￥1960
