- ￥3250
- biorbyt
- orb764232
- 英国
- 2025年12月05日
- ELISA, IHC-P, WB
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
MEK-3 (phospho Ser218) rabbit pAb抗体
- 抗体英文名：
MEK-3 (phospho Ser218) rabbit pAb
- 浓度：
1 mg/ml
- 应用范围：
ELISA, IHC-P, WB
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb764232
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 亚型：
IgG
- 免疫原：
The antiserum was produced against synthesized peptide derived from human MKK3 around the phosphorylation site of Ser189. AA range:173-222
- 规格：
50 ul
别名:Anti-MAP2K3 antibody, anti-MEK3 antibody, anti-MKK3 antibody, anti-PRKMK3 antibody, anti-SKK2 antibody, anti-Dual specificity mitogen-activated protein kinase kinase 3 antibody, anti-MAP kinase kinase 3 antibody, anti-MAPKK 3 antibody, anti-MAPK/ERK kinase 3 antibody, anti-MEK 3 antibody, anti-Stress-activated protein kinase kinase 2 antibody, anti-SAPK kin antibody
免疫原：The antiserum was produced against synthesized peptide derived from human MKK3 around the phosphorylation site of Ser189. AA range:173-222
分子量:39
应用稀释比例：Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
防腐剂:PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
纯化:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
来源:Rabbit
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Note:For research use only.
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
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