- ￥2600
- biorbyt
- orb704341
- 英国
- 2025年12月05日
- FC, ICC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
Cleaved PARP Recombinant Rabbit Monoclonal Antibody抗体
- 抗体英文名：
Cleaved PARP Recombinant Rabbit Monoclonal Antibody
- 靶点：
PARP1
- 浓度：
1mg/ml
- 应用范围：
FC, ICC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb704341
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Recombinant
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
A synthesized peptide derived from human PARP 1 (214-250aa)
- 规格：
25 ul
产品描述：Cleaved PARP Recombinant Rabbit Monoclonal Antibody
别名:ADP ribosyltransferase(NAD+; poly(ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP1; PARP-1; Poly(ADP ribose) polymerase 1; poly(ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN.
免疫原：A synthesized peptide derived from human PARP 1 (214-250aa)
克隆性:2A10
分子量:89/113 kDa
应用稀释比例：WB=1:500-2000, Flow-Cyt=1:50-100, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P09874
Note:For research use only.
别名:ADP ribosyltransferase(NAD+; poly(ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP1; PARP-1; Poly(ADP ribose) polymerase 1; poly(ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN.
免疫原：A synthesized peptide derived from human PARP 1 (214-250aa)
克隆性:2A10
分子量:89/113 kDa
应用稀释比例：WB=1:500-2000, Flow-Cyt=1:50-100, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P09874
Note:For research use only.
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Cleaved PARP antibody (orb704341), dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Human THP-1 cell lysates, Lane 3: Human HL60 cell lysates, Lane 4: Human K562 cell lysates, Primary: Anti-Cleaved PARP (orb704341) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 89/116 kDa, Observed band size: 100 kDa.
Sample: Lane 1: Jurkat cell lysate, Lane 2: A549 cell lysate, Primary: Anti-Cleaved PARP (orb704341) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 89/110 kD, Observed band size: 89 kD.
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Cleaved PARP Recombinant Rabbit Monoclonal Antibody抗体，orb704341，biorbyt
￥2600
