- ￥1560
- biorbyt
- orb214324
- 英国
- 2025年12月04日
- IF, IHC, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
iNOS Antibody抗体
- 抗体英文名：
iNOS Antibody
- 应用范围：
IF, IHC, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb214324
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human iNOS. The exact sequence is proprietary.
- 规格：
30 ul
产品描述：The iNOS Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human iNOS. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
别名:NOS2A; Nitric oxide synthase, inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human iNOS. The exact sequence is proprietary.
应用稀释比例：WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:38782114
Entrez:18126, 24599, 4843
UniProt ID:Q06518, P35228, P29477
Note:For research use only.
别名:NOS2A; Nitric oxide synthase, inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human iNOS. The exact sequence is proprietary.
应用稀释比例：WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:38782114
Entrez:18126, 24599, 4843
UniProt ID:Q06518, P35228, P29477
Note:For research use only.
Western blot analysis of iNOS expression in MCF7 (A), A549 (B), Raw264.7 (C), PC12 (D), rat liver (E) whole cell lysates. (Predicted band size: 126; 131 kD; Observed band size: 130 kD)
Immunohistochemical analysis of iNOS staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of iNOS staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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文献和实验该产品被引用文献
Xuejing Hou 1, Shanshan Xiao 2, Xiaohong Xu 3, Mingze Qin 4, Xuebing Cheng 4, Xiangping Xu Glycoprotein Non-metastatic Melanoma Protein B (GPNMB) Protects Against Neuroinflammation and Neuronal Loss in Pilocarpine-induced Epilepsy via the Regulation of Microglial Polarization Neuroscience, (2024)
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iNOS Antibody抗体，orb214324，biorbyt
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