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Beta-1 Adrenergic Receptor Ant

ibody抗体,orb318975,biorbyt
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  • ¥1560
  • biorbyt已认证
  • orb318975
  • 英国
  • 2025年12月05日
  • IF, IHC, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Beta-1 Adrenergic Receptor Antibody抗体

    • 抗体英文名

      Beta-1 Adrenergic Receptor Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb318975

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Beta-1 Adrenergic Receptor. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The Beta-1 Adrenergic Receptor Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Beta-1 Adrenergic Receptor. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:ADRB1R; B1AR; Beta-1 adrenergic receptor; Beta-1 adrenoreceptor; Beta-1 adrenoceptor

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Beta-1 Adrenergic Receptor. The exact sequence is proprietary.

    应用稀释比例:WB: 1:500-1:1000, IHC-P: 1:100-1:200, IF/ICC: 1:100-1:500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:153

    UniProt ID:P08588

    Note:For research use only.

    产品细节图片1
    Western blot analysis of Beta-1 Adrenergic Receptor expression in mouse heart (A), rat heart (B), rat kidney (C) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 51 kD)

    产品细节图片2
    Immunohistochemical analysis of Beta-1 Adrenergic Receptor staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of Beta-1 Adrenergic Receptor staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    图标文献和实验
    相关实验
    • Isolation of Adrenergic Receptor Genes

      In order to isolate a single gene, phage or cosmid libraries can be screened by the conventional technique of hybridization as described by Sambrook et al. (1 ) using end-labeled oligonucleotide probes or gene-specific probes. The probes

    • Analyses of Adrenergic Receptor Sequences

      with known adrenergic receptor sequences in order to verify the receptor and subtype, to submit the sequence to GenBank, and to analyze the predicted secondary structure of the receptor.

    • Targeted Disruption of Adrenergic Receptor Genes

      . This approach has been particularly useful for studies on adrenergic receptor (AR) function, having been used to effect disruption of at least six of the nine known AR genes (1 –6 ). As presented here, the knockout strategy is essentially a synthesis

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