- ￥1560
- biorbyt
- orb378044
- 英国
- 2025年12月05日
- IF, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
EIF3A Antibody抗体
- 抗体英文名：
EIF3A Antibody
- 应用范围：
IF, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb378044
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原：
Recombinant fusion protein of human EIF3A
- 规格：
30 ul
产品描述：The EIF3A Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human EIF3A .Purification: The antibody was purified by immunogen affinity chromatography.
别名:EIF3S10; KIAA0139; Eukaryotic translation initiation factor 3 subunit A; eIF3a; Eukaryotic translation initiation factor 3 subunit 10; eIF-3-theta; eIF3 p167; eIF3 p180; eIF3 p185
免疫原：Recombinant fusion protein of human EIF3A
应用稀释比例：WB: 1:500-1000, IHC-P: 1:50-200
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:8661, 13669, 292148
UniProt ID:Q14152, P23116, Q1JU68
Note:For research use only.
别名:EIF3S10; KIAA0139; Eukaryotic translation initiation factor 3 subunit A; eIF3a; Eukaryotic translation initiation factor 3 subunit 10; eIF-3-theta; eIF3 p167; eIF3 p180; eIF3 p185
免疫原：Recombinant fusion protein of human EIF3A
应用稀释比例：WB: 1:500-1000, IHC-P: 1:50-200
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:8661, 13669, 292148
UniProt ID:Q14152, P23116, Q1JU68
Note:For research use only.
Western blot analysis of EIF3A expression in HepG2 (A), Jurkat (B), mouse heart (C), mouse ovary (D) whole cell lysates. (Predicted band size: 162; 166 kD; Observed band size: 180 kD)
Immunofluorescent analysis of EIF3A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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文献和实验相关实验
Nat Commun：肖东/孙妍课题组合作揭示 m6A 修饰调节细胞自噬的新机制
的 m6A 修饰位点，促进 FOXO3 翻译。另外，YTHDF3 还可募集翻译起始因子 eIF3a 和 eIF4B 以促进 FOXO3 翻译，进而启动自噬。 图 3 YTHDF3 识别饥饿诱导的 FOXO3 mRNA 的 m6A 超甲基化 （图源：Hao WC, et al., Nat Commun, 2022） 综上，该研究揭示了表观转录修饰和自噬调节之间的重要联系。所提供的研究证据表明，m6A 阅读器 YTHDF3 可通过识别 METTL3 介导的 m6A 高甲基化发挥营养反应器的作用来诱导
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EIF3A Antibody抗体，orb378044，biorbyt
￥1560
