- ￥1820
- biorbyt
- orb216026
- 英国
- 2025年12月05日
- IF, IHC, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
ATF4 (Phospho-S245) Antibody抗体
- 抗体英文名：
ATF4 (Phospho-S245) Antibody
- 应用范围：
IF, IHC, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb216026
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原：
KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S245 of human ATF4 protein. The exact sequence is proprietary.
- 规格：
30 ul
产品描述：The ATF4 (Phospho-S245) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S245 of human ATF4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
别名:CREB2; TXREB; Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67
免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S245 of human ATF4 protein. The exact sequence is proprietary.
应用稀释比例：WB: 1:500-1:1000, IHC-P: 1:100-1-200
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:11911, 79255, 468
UniProt ID:Q06507, P18848, Q9ES19
Note:For research use only.
别名:CREB2; TXREB; Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67
免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S245 of human ATF4 protein. The exact sequence is proprietary.
应用稀释比例：WB: 1:500-1:1000, IHC-P: 1:100-1-200
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:11911, 79255, 468
UniProt ID:Q06507, P18848, Q9ES19
Note:For research use only.
Western blot analysis of ATF4 (Phospho-S245) expression in SGC7901 (A), 3T3L1 (B), C6 (C), EC9706 (D), A375 (E) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 55 kD)
Immunohistochemical analysis of ATF4 (Phospho-S245) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ATF4 (Phospho-S245) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Direct ELISA antibody dose-response curve using Anti-ATF4 (Phospho-S245) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
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ATF4 (Phospho-S245) Antibody抗体，orb216026，biorbyt
￥1820
