p53 (Phospho-S15) Rabbit Polyclonal Antibody 抗体,orb214691,Biorbyt产品图

p53 (Phospho-S15) Rabbit Polyc

lonal Antibody 抗体,orb214691,Biorbyt
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  • ¥1872 - 5148
  • Biorbyt已认证
  • orb214691
  • 英国
  • 2026年07月28日
  • IF, IHC, IP, WB
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      p53 (Phospho-S15) Rabbit Polyclonal Antibody 抗体

    • 抗体英文名

      p53 (Phospho-S15) Rabbit Polyclonal Antibody

    • 靶点

      TP53

    • 应用范围

      IF, IHC, IP, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb214691

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Liquid

    • 免疫原

      详询

    • 规格

      30 ul/50 ul/100 ul/200 ul

    规格:30 ul产品价格:¥1872.0
    规格:50 ul产品价格:¥2340.0
    规格:100 ul产品价格:¥3627.0
    规格:200 ul产品价格:¥5148.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: The p53 (Phospho-S15) Antibody is suitable for IF, IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S15 of human p53 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名: P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

    应用稀释比例: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100

    免疫原: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S15 of human p53 protein. The exact sequence is proprietary.

    防腐剂: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    纯化方法: The antibody was purified by immunogen affinity chromatography.

    特异性: Recognizes endogenous levels of p53 protein only when phosphorylated at S15.

    研究领域: Cancer Biology

    UniProt ID: P10361, P04637

    Entrez 基因 ID: 24842, 7157

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of p53 (Phospho-S15) expression in Hela (A), Jurkat (B) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 53; 47 kD)

    产品细节图片2
    Immunohistochemical analysis of p53 (Phospho-S15) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of p53 (Phospho-S15) staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

    产品细节图片4
    Direct ELISA antibody dose-response curve using Anti-p53 (Phospho-S15) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

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    图标文献和实验
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    ¥1872 - 5148