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Topoisomerase 2 alpha Antibody

[KO/KD Validated]抗体，orb214689，biorbyt
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  • ￥1560
  • biorbyt已认证
  • orb214689
  • 英国
  • 2025年12月05日
  • IF, IHC, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      Topoisomerase 2 alpha Antibody [KO/KD Validated]抗体

    • 抗体英文名：

      Topoisomerase 2 alpha Antibody [KO/KD Validated]

    • 应用范围：

      IF, IHC, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb214689

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 形态：

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原：

      KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Topoisomerase 2 alpha. The exact sequence is proprietary.

    • 规格：

      30 ul

    产品描述：The Topoisomerase 2 alpha Antibody [KO/KD Validated] is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Topoisomerase 2 alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme

    免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Topoisomerase 2 alpha. The exact sequence is proprietary.

    应用稀释比例：WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:7153

    UniProt ID:P11388

    Note:For research use only.

    产品细节图片1
    Western blot analysis of Topoisomerase 2 alpha expression in Hela (A), H446 (B) whole cell lysates. (Predicted band size: 174 kD; Observed band size: 190 kD)

    产品细节图片2
    Immunohistochemical analysis of Topoisomerase 2 alpha staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of Topoisomerase 2 alpha staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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