人EGFR重组蛋白/Human EGFR(313-445)

产品类型	ECD Proteins
靶点	EGFR
Uniprot ID	P00533
别名	ERBB, ERRP, HER1, mENA, ERBB1, NNCIS, PIG61, NISBD2
背景	The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). [provided by RefSeq, Jul 2020]
产品英文名称	Recombinant human EGFR(313-445) Protein with C-terminal human Fc tag
分子结构	EGFR(Ala313-Ile445) hFc(Glu99-Ala330)
分子量	The protein has a predicted molecular mass of 41.0 kDa after removal of the signal peptide.
纯度	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
标签	C-Human Fc tag
有无偶联	Unconjugated
表达宿主	HEK293
组分	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
货期	In Stock
储存与运输	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
应用范围	Research use only

Figure 1. Human EGFR(313-445) Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human EGFR(313-445) Protein, hFc Tag (PME101867) can bind Anti-EGFR(depatuxizumab biosimilar) mAb (BME100252) in a linear range of 200–1000 ng/mL.In order to specifically detect BME100252, mouse anti-human Fab-specific antibody was used as detection antibody.