- ￥4160
- biorbyt
- orb559083
- 英国
- 2025年12月04日
- ICC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody抗体
- 抗体英文名：
Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody
- 靶点：
KRT1
- 浓度：
1mg/ml
- 应用范围：
ICC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb559083
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Recombinant
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
A synthesized peptide derived from human Cytokeratin 1 (600-644aa)
- 规格：
50 ul
产品描述：Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody
别名:Cytokeratin-1; 67 kDa cytokeratin; CK 1; CK1; Cytokeratin1; EHK1; Hair alpha protein; K 1; K1; Keratin 1; Keratin type II cytoskeletal 1; Keratin1; KRT 1; KRT1A; K2C1_HUMAN; Keratin, type II cytoskeletal 1; Cytokeratin-1; CK-1; Keratin-1; Type-II keratin Kb1.
免疫原：A synthesized peptide derived from human Cytokeratin 1 (600-644aa)
预测反应性:Mouse, Rat
克隆性:1C2
分子量:70 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P04264
Note:For research use only.
别名:Cytokeratin-1; 67 kDa cytokeratin; CK 1; CK1; Cytokeratin1; EHK1; Hair alpha protein; K 1; K1; Keratin 1; Keratin type II cytoskeletal 1; Keratin1; KRT 1; KRT1A; K2C1_HUMAN; Keratin, type II cytoskeletal 1; Cytokeratin-1; CK-1; Keratin-1; Type-II keratin Kb1.
免疫原：A synthesized peptide derived from human Cytokeratin 1 (600-644aa)
预测反应性:Mouse, Rat
克隆性:1C2
分子量:70 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P04264
Note:For research use only.
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (orb559083) and Vimentin. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (orb559083, red) at 1/200 dilution and Vimentin (green) at 1/200 dilution at +4°C overnight, washed with PBS.
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Cytokeratin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb559083, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb559083, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Cytokeratin 1 antibody (orb559083) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb559083) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Cytokeratin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb559083, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, Lane 2: 293T cell lysate.
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Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody抗体，orb559083，biorbyt
￥4160
