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ERK1/2 Mouse Monoclonal Antibo

dy抗体，orb500888，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb500888
  • 英国
  • 2025年12月04日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Mouse
  • Human, Mouse, Rat
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      ERK1/2 Mouse Monoclonal Antibody抗体

    • 抗体英文名：

      ERK1/2 Mouse Monoclonal Antibody

    • 靶点：

      MAPK3

    • 浓度：

      1mg/ml

    • 应用范围：

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主：

      Mouse

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb500888

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Monoclonal

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated synthetic peptide derived from human ERK1/2 (151-250/380aa)

    • 规格：

      50 ul

    产品描述：ERK1/2 Mouse Monoclonal Antibody

    别名:ERK; ERK-1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; Insulin Stimulated MAP2 Kinase; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK; MAPK1; Mapk3; MGC20180; Microtubule Associated Protein 2 Kinase; Microtubule-associated protein 2 kinase; Mitogen Activated Protein Kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03_HUMAN; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM 3; PRKM3; Protein Kinase Mitogen Activated 3.

    免疫原：KLH conjugated synthetic peptide derived from human ERK1/2 (151-250/380aa)

    预测反应性:Mouse, Rat

    克隆性:3G4

    分子量:43 kDa

    应用稀释比例：WB=1:500-5000, IHC-P=1:500-2000, IHC-F=1:500-2000, IF=1:500-2000, Flow-Cyt=1μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein G

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P27361

    Note:For research use only.

    产品细节图片1
    paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of orb500888) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

    产品细节图片2
    Fixative-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of orb500888) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of orb500888) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

    产品细节图片4
    Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: SiHa (Human) Cell Lysate at 30 ug, Lane 5: MCF-7 (Human) Cell Lysate at 30 ug, Lane 6: HUVEC (Human) Cell Lysate at 30 ug, Primary: Anti-ERK1/2 (orb500888) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 44/42 kD, Observed band size: 40/42 kD.

    产品细节图片5
    Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-ERK1/2 (orb500888) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 38 kD.

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