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Phospho-PKC delta (Tyr52) Rabb

it Polyclonal Antibody抗体,orb6749,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb6749
  • 英国
  • 2025年12月04日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Phospho-PKC delta (Tyr52) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-PKC delta (Tyr52) Rabbit Polyclonal Antibody

    • 靶点

      PRKCD

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb6749

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human PKC delta around the phosphorylation site of Tyr52 TM(p-Y)PE

    • 规格

      50 ul

    产品描述:Phospho-PKC delta (Tyr52) Rabbit Polyclonal Antibody

    别名:PKC delta(phospho Tyr52); PKC delta(phospho Y52); MAY 1; MAY1; nPKC delta; PCKd; PKC d; PKC delta; PKC-d; PKCD; PKCdelta; PRKC D; PRKC delta; PRKC-d; PRKCd; Protein Kinase C delta; Protein kinase C delta type; Protein Kinase Cdelta; KPCD_HUMAN; PKC δ.

    免疫原:KLH conjugated Synthesised phosphopeptide derived from human PKC delta around the phosphorylation site of Tyr52 TM(p-Y)PE

    预测反应性:Bovine, Canine, Porcine, Rabbit

    分子量:77 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:Q05655

    Note:For research use only.

    产品细节图片1
    Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-phospho-PKC delta (Tyr52) antibody (orb6749), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): F (ab')2 fragment goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 2% Fixative (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Fixative-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PKC delta (Tyr52)) Polyclonal Antibody, Unconjugated (orb6749) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PKC delta (Tyr52)) Polyclonal Antibody, Unconjugated (orb6749) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Sample: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-phospho-PKC delta (Tyr52) (orb6749) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 77 kD, Observed band size: 80 kD.

    产品细节图片5
    Tissue/Cell: mouse brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-PKC delta (Tyr52) Polyclonal Antibody, Unconjugated (orb6749) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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