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Phospho-GSK3 Beta (Tyr216) Rab

bit Polyclonal Antibody抗体,orb5359,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb5359
  • 英国
  • 2025年12月04日
  • WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Phospho-GSK3 Beta (Tyr216) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-GSK3 Beta (Tyr216) Rabbit Polyclonal Antibody

    • 靶点

      GSK3B

    • 浓度

      1mg/ml

    • 应用范围

      WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb5359

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216 VS(p-Y)IC

    • 规格

      50 ul

    产品描述:Phospho-GSK3 Beta (Tyr216) Rabbit Polyclonal Antibody

    别名:GSK3 beta(phospho Y216); p-GSK3 beta(phospho Y216); GSK3B(phospho-Tyr216); GSK3B(phospho-Y216); p-GSK-3 beta(Tyr216); p-GSK-3 beta(Y216); Glycogen synthase kinase 3 beta; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B_HUMAN.

    免疫原:KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216 VS(p-Y)IC

    预测反应性:Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep

    分子量:47 kDa

    应用稀释比例:WB=1:500-2000

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P49841

    Note:For research use only.

    产品细节图片1
    paraffin embedded (human pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (phospho-GSK3 Beta (Tyr216)) Polyclonal Antibody, Unconjugated (orb5359) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片2
    Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Mouse NIH/3T3 cell lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Testis tissue lysates, Primary: Anti-phospho-GSK3 Beta (Tyr216) (orb5359) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 50 kDa.

    产品细节图片3
    Sample: Testis (Mouse) Lysate at 40 ug, Thymus (Mouse) Lysate at 40 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-GSK3 Beta (Tyr216) (orb5359) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 49 kD.

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    图标文献和实验
    相关实验
    • 【求助】关于gsk3-beta功能的疑问

      的关系理不出头绪。GSK-3 beta 总蛋白减少,p-GSK-3 beta (ser 9)增加,是不是足以说明有活性的GSK-3 beta 减少更多,这样理解不知道对不对,希望给以指点。 galaxy22 楼主你好,请问你做出结果的tyr216抗体是哪个公司的。。。我做出来总量是减少的,active B catenin是减少的,那我有必要做ser9 和tyr216的磷酸化形式吗?我想研究wnt和akt。。。 棉花糖的温柔

    • v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平

      loop of the kinase domain (Tyr279 in GSK3a, andTyr216 in GSK3b), which is absolutely required for kinase activity. Thirdly, phosphorylation of GSK3 at a conserved N-terminalserine residue inhibits its kinase activity. This phosphoserine actsas

    • v468 chapter 5 糖原合成酶激酶-3 的抑制

      antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San

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