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SHANK Antibody (HRP)抗体,orb1485

88,biorbyt
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  • ¥8320
  • biorbyt已认证
  • orb148588
  • 英国
  • 2025年12月04日
  • AM, ICC, IF, IHC, IP, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      SHANK Antibody (HRP)抗体

    • 抗体英文名

      SHANK Antibody (HRP)

    • 靶点

      SHANK

    • 浓度

      1 mg/ml

    • 应用范围

      AM, ICC, IF, IHC, IP, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb148588

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      HRP

    • 克隆性

      Monoclonal

    • 亚型

      IgG1

    • 免疫原

      Fusion protein amino acids 84-309 of rat Shank2

    • 规格

      100 ug

    产品描述:Mouse monoclonal to SHANK (HRP). Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins...

    别名:Cortactin binding protein 1, Cortactin SH3 domain-binding protein, Cortactin-binding protein 1, CortBP1, CTTNBP1, GKAP/SAPAP interacting protein, GKAP/SAPAP-interacting protein, KIAA1022, Proline rich synapse associated protein 1, Proline-rich synapse-associated protein 1, ProSAP1, SH3 and multiple ankyrin repeat domains protein 2, SHAN2_RAT, Shank2, SPANK-3

    免疫原:Fusion protein amino acids 84-309 of rat Shank2

    克隆性:N23b/49 (Formerly sold as S23b-49)

    分子量:160kDa

    应用稀释比例:WB (1:1000), IHC (1:1000), ICC/IF (1:100)

    应用注释:1 µg/ml of SMC-327 was sufficient for detection of Shank1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    防腐剂:73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

    纯化:Protein G Purified

    保存说明:Conjugated antibodies should be stored according to the product label

    Entrez:171093

    UniProt ID:Q9QX74

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK (pan) Antibody (D) Composite.

    产品细节图片2
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK (pan) Antibody. (D) Composite.

    产品细节图片3
    Western Blot analysis of Rat brain membrane lysate showing detection of SHANK protein using Mouse Anti-SHANK Monoclonal Antibody, Clone S23b-49. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

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    图标文献和实验
    相关实验
    • Conjugation of Antibody to HRP

      of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator.  Desalt the HRP on PBS equilibrated column.  Collect

    • Conjugation of Antibody to HRP

        Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki     Reagents

    • Monoclonal Antibody Production Protocol

      - PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase

    图标技术资料

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