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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
SHANK Antibody (HRP)抗体
- 抗体英文名:
SHANK Antibody (HRP)
- 靶点:
SHANK
- 浓度:
1 mg/ml
- 应用范围:
AM, ICC, IF, IHC, IP, WB
- 宿主:
Mouse
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb148588
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
HRP
- 克隆性:
Monoclonal
- 亚型:
IgG1
- 免疫原:
Fusion protein amino acids 84-309 of rat Shank2
- 规格:
100 ug
别名:Cortactin binding protein 1, Cortactin SH3 domain-binding protein, Cortactin-binding protein 1, CortBP1, CTTNBP1, GKAP/SAPAP interacting protein, GKAP/SAPAP-interacting protein, KIAA1022, Proline rich synapse associated protein 1, Proline-rich synapse-associated protein 1, ProSAP1, SH3 and multiple ankyrin repeat domains protein 2, SHAN2_RAT, Shank2, SPANK-3
免疫原:Fusion protein amino acids 84-309 of rat Shank2
克隆性:N23b/49 (Formerly sold as S23b-49)
分子量:160kDa
应用稀释比例:WB (1:1000), IHC (1:1000), ICC/IF (1:100)
应用注释:1 µg/ml of SMC-327 was sufficient for detection of Shank1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
防腐剂:73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
纯化:Protein G Purified
保存说明:Conjugated antibodies should be stored according to the product label
Entrez:171093
UniProt ID:Q9QX74
Note:For research use only.



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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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