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SHANK Antibody抗体,orb67415,bior

byt
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  • ¥7670
  • biorbyt已认证
  • orb67415
  • 英国
  • 2025年12月04日
  • AM, ICC, IF, IHC, IP, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      SHANK Antibody抗体

    • 抗体英文名

      SHANK Antibody

    • 靶点

      SHANK

    • 浓度

      1 mg/ml

    • 应用范围

      AM, ICC, IF, IHC, IP, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb67415

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Monoclonal

    • 亚型

      IgG1

    • 免疫原

      Fusion protein amino acids 84-309 of rat Shank2

    • 规格

      100 ug

    产品描述:Mouse Anti-Rat SHANK (pan) Monoclonal IgG1

    别名:Cortactin binding protein 1, Cortactin SH3 domain-binding protein, Cortactin-binding protein 1, CortBP1, CTTNBP1, GKAP/SAPAP interacting protein, GKAP/SAPAP-interacting protein, KIAA1022, Proline rich synapse associated protein 1, Proline-rich synapse-associated protein 1, ProSAP1, SH3 and multiple ankyrin repeat domains protein 2, SHAN2_RAT, Shank2, SPANK-3

    免疫原:Fusion protein amino acids 84-309 of rat Shank2

    克隆性:N23b/49 (Formerly sold as S23b-49)

    分子量:160kDa

    应用稀释比例:WB (1:1000), IHC-P (1:1000), ICC/IF (1:100)

    应用注释:1 µg/ml of SMC-327 was sufficient for detection of Shank1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    防腐剂:PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

    纯化:Protein G Purified

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:171093

    UniProt ID:Q9QX74

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK (pan) Antibody (D) Composite.

    产品细节图片2
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK (pan) Antibody. (D) Composite.

    产品细节图片3
    Western Blot analysis of Rat brain membrane lysate showing detection of SHANK protein using Mouse Anti-SHANK Monoclonal Antibody, Clone S23b-49. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

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    图标文献和实验
    相关实验
    • ES injection pipette manufacture(图)

      diameter of 1.0 mm and an internal diameter of 0.8 mm. It is NON FILAMENTED. Using a Kopf Model 750 vertical puller, I draw out glass such that it has a long shank of similar diameter. This will give me the advantage of several attempts to cut the glass

    • ES injection pipette manufacture

      listing). This glass has an external diameter of 1.0 mm and an internal diameter of 0.8 mm. It is NON FILAMENTED. Using a Kopf Model 750 vertical puller, I draw out glass such that it has a long shank of similar diameter

    • Method for Single-Cell Electroporation

      a P-87 Micropipette Puller. In general, a patch-clamp type electrode may be sufficient. The tip size should be around 0.6-1 µm and have a resistance about 10 M when filled with standard intracellular recording solution. Shank dimensions can vary

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