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CLEC 4E Rabbit Polyclonal Anti

body抗体，orb1341，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb1341
  • 英国
  • 2025年12月04日
  • FC
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      CLEC 4E Rabbit Polyclonal Antibody抗体

    • 抗体英文名：

      CLEC 4E Rabbit Polyclonal Antibody

    • 靶点：

      CLEC4E

    • 浓度：

      1mg/ml

    • 应用范围：

      FC

    • 宿主：

      Rabbit

    • 适应物种：

      Human

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb1341

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated synthetic peptide derived from human CLECSF9 (51-150/219aa)

    • 规格：

      50 ul

    产品描述：CLEC 4E Rabbit Polyclonal Antibody

    别名:C type lectin domain family 4 member E; C type lectin superfamily member 9; C-type(calcium dependent carbohydrate recognition domain) lectin superfamily member 9; C-type lectin domain family 4 member E; C-type lectin superfamily member 9; CLC4E_HUMAN; CLEC 4E; CLEC4E Clec4e; CLECSF9; Macrophage inducible C type lectin; Macrophage-inducible C-type lectin; MINCLE.

    免疫原：KLH conjugated synthetic peptide derived from human CLECSF9 (51-150/219aa)

    预测反应性:Bovine, Equine, Mouse, Rat

    分子量:25 kDa

    应用稀释比例：Flow-Cyt=2μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:Q9ULY5

    Note:For research use only.

    产品细节图片1
    Black line: Positive blank control (Hela), Negative blank control (A431), Green line: Primary Antibody (Rabbit Anti-CLEC 4E antibody (orb1341)), Orange line: Isotype Control Antibody (Rabbit IgG). Blue line: Secondary Antibody (Goat anti-rabbit IgG-PE)/ (Goat anti-rabbit IgG-AF488), Hela (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CLEC 4E Antibody (orb1341) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

    产品细节图片2
    Blank control: Hela (blue), the cells were fixed with 2% Fixative (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA (green).

    产品细节图片3
    Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CLEC 4E antibody (orb1341), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

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