RAD21 Rabbit Polyclonal Antibody抗体,orb100862,biorbyt

RAD21 Rabbit Polyclonal Antibo

dy抗体,orb100862,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb100862
  • 英国
  • 2025年12月04日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      RAD21 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      RAD21 Rabbit Polyclonal Antibody

    • 靶点

      RAD21

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb100862

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human Rad21 (531-631/631aa)

    • 规格

      50 ul

    产品描述:RAD21 Rabbit Polyclonal Antibody

    别名:Double-strand-break repair protein rad21 homolog; hHR21; HR21; HRAD21; MCD1; Nuclear matrix protein 1; NXP-1; NXP1; RAD21; RAD21 homolog; RAD21_HUMAN; Scc1; SCC1 homolog; FLJ25655; FLJ40596; KIAA0078; SCC1.

    免疫原:KLH conjugated synthetic peptide derived from human Rad21 (531-631/631aa)

    预测反应性:Bovine, Canine, Equine, Gallus, Mouse, Porcine

    分子量:69 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:O60216

    Note:For research use only.

    RAD21 Rabbit Polyclonal Antibo
    Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-RAD21 antibody, Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, Dilution: 3 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    RAD21 Rabbit Polyclonal Antibo
    Tissue/cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-RAD21 Polyclonal Antibody, Unconjugated (orb100862) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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