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Phospho-HSP70 (Tyr525) Rabbit

Polyclonal Antibody抗体,orb5488,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb5488
  • 英国
  • 2025年12月04日
  • FC, ICC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Phospho-HSP70 (Tyr525) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-HSP70 (Tyr525) Rabbit Polyclonal Antibody

    • 靶点

      HSPA1A

    • 浓度

      1mg/ml

    • 应用范围

      FC, ICC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb5488

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr525 EK(p-Y)KA

    • 规格

      50 ul

    产品描述:Phospho-HSP70 (Tyr525) Rabbit Polyclonal Antibody

    别名:HSP70(phospho Tyr525); HSP70(phospho Y525); p-HSP70(Tyr525); p-HSP70(Y525); HSP70; HSP-70; HSP 70; Heat shock 70 kDa protein 1; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; HSP70.1; HSP72; HSPA1; HSPA1A; HSPA1B; XXbac BCX40G17.3 001; Heat shock 70 kDa protein 1A/1B; HSP71A_HUMAN; Hspa1a; Hsp70-1; HSP72; Hspa1; Hspa1b; Heat shock 70 kDa protein 1A/1B; shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2.

    免疫原:KLH conjugated Synthesised phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr525 EK(p-Y)KA

    预测反应性:Bovine, Canine, Gallus, Guinea pig, Mouse, Porcine, Rabbit, Sheep

    分子量:70 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:22899526

    UniProt ID:P0DMV8

    Note:For research use only.

    产品细节图片1
    Blank control (blue line): Jurkat (fixed with 2% Fixative (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-HSP70 (Tyr525) antibody (orb5488), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

    产品细节图片2
    Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-HSP70 (Tyr525)) polyclonal Antibody, Unconjugated (orb5488) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片3
    Fixative-fixed, paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-HSP70 (Tyr525)) Polyclonal Antibody, Unconjugated (orb5488) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (Human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-HSP70 (Tyr525)) Polyclonal Antibody, Unconjugated (orb5488) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

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    图标文献和实验
    该产品被引用文献
    Jammes, Yves et al. Fatiguing stimulation of one skeletal muscle triggers heat shock protein activation in several rat organs: the role of muscle innervation J Exp Biol, 215, 4041-4048 (2012)
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    文献支持
    Phospho-HSP70 (Tyr525) Rabbit Polyclonal Antibody抗体,orb5488,biorbyt
    ¥3120