- ￥3120
- biorbyt
- orb10547
- 英国
- 2025年12月04日
- FC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
Dnmt3a Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Dnmt3a Rabbit Polyclonal Antibody
- 靶点：
DNMT3A
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb10547
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
参考产品说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human Dnmt3a (26-100/912aa)
- 规格：
50 ul
产品描述：Dnmt3a Rabbit Polyclonal Antibody
别名:DNA(cytosine 5 ) methyltransferase 3 alpha; DNA(cytosine 5) methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaI; DNA MTase HsaIIIA; DNMT 3a; DNMT; DNMT3A2; M HsaIIIA; M.HsaIIIA; MCMT; Methyl CpG binding domain protein 3a.
免疫原：KLH conjugated synthetic peptide derived from human Dnmt3a (26-100/912aa)
预测反应性:Bovine
分子量:100 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q9Y6K1
Note:For research use only.
别名:DNA(cytosine 5 ) methyltransferase 3 alpha; DNA(cytosine 5) methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaI; DNA MTase HsaIIIA; DNMT 3a; DNMT; DNMT3A2; M HsaIIIA; M.HsaIIIA; MCMT; Methyl CpG binding domain protein 3a.
免疫原：KLH conjugated synthetic peptide derived from human Dnmt3a (26-100/912aa)
预测反应性:Bovine
分子量:100 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q9Y6K1
Note:For research use only.
Blank control (Black line): U87MG (Black). Primary Antibody (green line): Rabbit Anti-Dnmt3a antibody (orb10547), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (rat kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Dnmt3a) Polyclonal Antibody, Unconjugated (orb10547) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: A549 (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Lovo (Human) Cell Lysate at 30 ug, Primary: Anti-Dnmt3a (orb10547) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 120 kD.
Sample: Embryo (Mouse) Lysate at 40 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Dnmt3a (orb10547) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 120 kD.
Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 3: Testis (Rat) Lysate at 40 ug, Lane 4: 293T (Human) Cell Lysate at 30 ug, Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 6: Molt-4 (Human) Cell Lysate at 30 ug, Lane 7: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-Dnmt3a (orb10547) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.
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Dnmt3a Rabbit Polyclonal Antibody抗体，orb10547，biorbyt
￥3120
