- ￥3120
- biorbyt
- orb10543
- 英国
- 2025年12月04日
- FC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
PARK7/DJ1 Rabbit Polyclonal Antibody抗体
- 抗体英文名：
PARK7/DJ1 Rabbit Polyclonal Antibody
- 靶点：
PARK7
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb10543
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
参考产品说明书
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human CAP1 (101-189/189aa)
- 规格：
50 ul
产品描述：PARK7/DJ1 Rabbit Polyclonal Antibody
别名:PARK7; PARK-7; Parkinson disease protein 7; Park7 protein; CAP1; DJ-1; DJ 1; SP22; Protein DJ-1; Oncogene DJ1; FLJ27376; Park 7; Parkinson disease(autosomal recessive early onset) 7; RNA binding protein regulatory subunit; RS; SP22; CAP1_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human CAP1 (101-189/189aa)
预测反应性:Bovine, Equine, Porcine
分子量:20 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q99497
Note:For research use only.
别名:PARK7; PARK-7; Parkinson disease protein 7; Park7 protein; CAP1; DJ-1; DJ 1; SP22; Protein DJ-1; Oncogene DJ1; FLJ27376; Park 7; Parkinson disease(autosomal recessive early onset) 7; RNA binding protein regulatory subunit; RS; SP22; CAP1_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human CAP1 (101-189/189aa)
预测反应性:Bovine, Equine, Porcine
分子量:20 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q99497
Note:For research use only.
Blank control: 293T cells (blue). Primary Antibody: Rabbit Anti-PARK7/CAP1 antibody (orb10543), Dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, Primary antibody (orb10543, 0.2 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PARK7) Polyclonal Antibody, Unconjugated (orb10543) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PARK7) Polyclonal Antibody, Unconjugated (orb10543) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: Thyroid gland Rat) Lysate at 40 ug, Lane 5: Kidney (Rat) Lysate at 40 ug, Lane 6: Liver (Rat) Lysate at 40 ug, Lane 7: Hela (Human) Cell Lysate at 30 ug, Lane 8: U937 (Human) Cell Lysate at 30 ug, Lane 9: K562 (Human) Cell Lysate at 30 ug, Lane 10: HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-PARK7/DJ1 (orb10543) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.
The blue histogram is unstained cells (Hepg2 cells), concentration 1:50, The Wathet Blue histogram is cells stained with secondary antibody alone. The Orange histogram is cells stained with rabbit IgG isotype control antibody, plus secondary antibody. The green histogram is cells stained with Rabbit Anti-PARK7/CAP1 antibody (orb10543) plus secondary antibody.
Tissue/Cell: rat kidney tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CAP1/PARK7 Polyclonal Antibody, Unconjugated (orb10543) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat kidney tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CAP1/PARK7 Polyclonal Antibody, Unconjugated (orb10543) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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PARK7/DJ1 Rabbit Polyclonal Antibody抗体，orb10543，biorbyt
￥3120
