- ￥1569 - 2169
- 亚科因(Abbkine)
- 湖北武汉
- KTB1641-C
- 2025年12月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃保存避光，6个月
- 保质期：
4℃保存避光，6个月
- 英文名：
Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit (DTNB Assay)
- 库存：
999
- 供应商：
亚科因(Abbkine)
- 规格：
48 T/48 S/96 T/96 S
| 规格： | 48 T/48 S | 产品价格： | ￥1569.0 |
|---|---|---|---|
| 规格： | 96 T/96 S | 产品价格： | ￥2169.0 |
谷胱甘肽过氧化物酶（GSH-Px）活性检测试剂盒（DTNB法）
产品概述
本试剂盒为Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit (DTNB Assay)，货号：KTB1641-C，专用于精准定量动植物组织、细胞、细菌、真菌及血清（浆）样本中的谷胱甘肽过氧化物酶（GSH-Px）活性。该产品基于经典的比色法（DTNB法）原理设计，适用于细胞代谢研究与氧化应激分析领域的高通量科研需求。
检测原理
谷胱甘肽过氧化物酶（GSH-Px）可催化还原型谷胱甘肽（GSH）与过氧化物反应，生成氧化型谷胱甘肽（GSSG），同时清除H₂O₂和脂质过氧化物，减轻氧化损伤。本试剂盒通过DTNB（5,5'-二硫代双-2-硝`基`苯甲酸）与GSH反应生成黄色产物，在412 nm波长下测定吸光度值，进而计算GSH-Px酶活性。
核心组分
- Extraction Buffer
- Reagent I
- Reagent II
- Reagent III
- Reagent Ⅳ
- Reagent Ⅴ
- Standard
技术参数
- 检测范围：6.25 - 400 nmol/mL
- 灵敏度：6.25 nmol/mL
- 样本类型：动植物组织、细胞裂解液、细菌/真菌提取物、血清（浆）
- 检测方法：比色法（DTNB法）
规格与交付
| 规格 | 价格（元） | 货期 |
|---|---|---|
| 48 T/48 S | 1569 | 现货（次日发货） |
| 96 T/96 S | 2169 | 现货（次日发货） |
使用建议
- 首次实验前建议进行预实验以优化样本量
- 样本均一化推荐使用BCA法测定蛋白浓度
- 需根据实验条件自行建立标准曲线
- 操作时需在通风环境下进行，并佩戴手套等防护用具
保存与运输
- 保存条件：4℃避光保存，有效期6个月
- 运输方式：蓝冰冰袋运输，保障试剂稳定性
注意事项
本品为生化试剂
应用领域
- 细胞氧化应激机制研究
- 抗氧化药物筛选评价
- 环境毒理学与食品安全检测
- 代谢性疾病相关基础研究
注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。
Glutathione Peroxidase (GSH-Px) Activity Assay Kit (DTNB Method)
Product Overview
This kit is the Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit (DTNB Assay), catalog number: KTB1641-C, specifically designed for the accurate quantification of glutathione peroxidase (GSH-Px) activity in samples including animal and plant tissues, cells, bacteria, fungi, and serum (plasma). The product is based on the classic colorimetric method (DTNB Assay) principle and is suitable for high-throughput research needs in the fields of cell metabolism studies and oxidative stress analysis.
Assay Principle
Glutathione peroxidase (GSH-Px) catalyzes the reaction between reduced glutathione (GSH) and peroxides, generating oxidized glutathione (GSSG), while clearing H₂O₂ and lipid peroxides to mitigate oxidative damage. This kit utilizes DTNB (5,5'-Dithiobis-(2-nitrobenzoic acid)) which reacts with GSH to produce a yellow-colored product. The absorbance is measured at 412 nm wavelength to calculate GSH-Px enzyme activity.
Core Components
- Extraction Buffer
- Reagent I
- Reagent II
- Reagent III
- Reagent Ⅳ
- Reagent Ⅴ
- Standard
Technical Parameters
- Detection Range: 6.25 - 400 nmol/mL
- Sensitivity: 6.25 nmol/mL
- Sample Types: Animal/plant tissues, cell lysates, bacterial/fungal extracts, serum (plasma)
- Assay Method: Colorimetric method (DTNB Assay)
Specifications & Delivery
| Specification | Price (¥) | Delivery Time |
|---|---|---|
| 48 T/48 S | 1569 | In stock (ships next day) |
| 96 T/96 S | 2169 | In stock (ships next day) |
Usage Recommendations
- A pilot experiment is recommended prior to the first use to optimize sample amount.
- Sample normalization is recommended using the BCA method for protein concentration determination.
- A standard curve should be established based on experimental conditions.
- Operations should be performed in a ventilated environment with protective equipment such as gloves.
Storage & Shipping
- Storage Conditions: Store protected from light at 4°C; valid for 6 months.
- Shipping Method: Shipped with blue ice packs to ensure reagent stability.
Precautions
This product is a biochemical reagent with certain irritancy and toxicity. Strictly adhere to laboratory safety protocols and avoid direct skin contact or inhalation.
Application Fields
- Research on cellular oxidative stress mechanisms
- Screening and evaluation of antioxidant drugs
- Environmental toxicology and food safety testing
- Basic research related to metabolic diseases
Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.
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