谷胱甘肽过氧化物酶活性检测试剂盒，用于对样本中GSH-Px活

谷胱甘肽过氧化物酶（GSH-Px）活性检测试剂盒

货号：KTB1640-C

产品类型：细胞代谢检测试剂盒

英文名称：Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit

产品概述

本试剂盒用于定量检测血清、血浆、动植物组织/细胞裂解液及其他生物体液样本中的谷胱甘肽过氧化物酶（GSH-Px）活性。采用比色法进行测定，适用于多种生物样本类型，并提供详尽的样本制备与结果计算指南。

检测原理

GSH-Px是谷胱甘肽氧化还原循环中的关键酶，可特异性催化还原型谷胱甘肽（GSH）与活性氧（ROS）反应，生成氧化型谷胱甘肽（GSSG），从而帮助维持细胞抗氧化稳态和机体免疫功能。其活性中心为硒半胱氨酸，因此GSH-Px活性也可作为体内硒水平的重要生化指标。

试剂盒组分

• Assay Buffer
• Substrate
• GR（谷胱甘肽还原酶）
• H₂O₂（过氧化氢）

特点与优势

• 适用于多种样本类型：血清、血浆、动植物组织及细胞裂解液等
• 提供完整的样本处理与活性计算方案
• 操作流程清晰，重复性良好

规格与价格

规格	价格（元）	货期
48 T/48 S	1569	现货（当天发货）
96 T/96 S	2169	现货（当天发货）

注意事项

• 需使用96孔UV微孔板进行检测
• 样本处理须在冰上操作，并于当日完成测定
• 细胞样本建议数目控制在3–5×10⁶之间，提取时使用Assay Buffer研磨或超声波处理，避免使用细胞裂解液
• 混合试剂需现配现用，并始终置于冰上
• 测定过程需迅速，空白管仅需设置1–2个

保存与运输

保存条件：-20℃避光保存，请参照说明书查看各组分具体存储要求。有效期为12个月（自发货日起）。

运输条件：蓝冰冰袋运输。

适用领域

本研究工具适用于细胞代谢、氧化应激、免疫调节及环境毒理学等领域的科学研究，不适用于人类或临床诊断。

警告：本产品仅限科研使用。使用前请确认不侵犯任何专利或许可证规定，厂家不对因使用本产品可能引发的专利侵权或其他违规行为承担责任。

别名：谷胱甘肽过氧化物酶检测试剂盒；GSH-Px活性检测盒；Glutathione Peroxidase Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Catalog Number: KTB1640-C

Product Type: Cell Metabolism Assay Kit

English Name: Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Product Overview

This kit is designed for the quantitative determination of glutathione peroxidase (GSH-Px) activity in serum, plasma, animal/plant tissue/cell lysates, and other biological fluid samples. It utilizes a colorimetric method, is suitable for various biological sample types, and provides detailed guidelines for sample preparation and result calculation.

Assay Principle

GSH-Px is a key enzyme in the glutathione redox cycle. It specifically catalyzes the reaction between reduced glutathione (GSH) and reactive oxygen species (ROS), producing oxidized glutathione (GSSG), thereby helping to maintain cellular antioxidant homeostasis and immune function. Its active center is selenocysteine, thus GSH-Px activity can also serve as an important biochemical indicator of selenium levels in vivo.

Kit Components

• Assay Buffer
• Substrate
• GR (Glutathione Reductase)
• H₂O₂ (Hydrogen Peroxide)

Features & Advantages

• Suitable for various sample types: serum, plasma, animal/plant tissues, and cell lysates
• Provides comprehensive sample processing and activity calculation protocols
• Clear procedure with good reproducibility

Specifications & Pricing

Specification	Price (RMB)	Delivery Time
48 T/48 S	1569	In Stock (Same-day Shipping)
96 T/96 S	2169	In Stock (Same-day Shipping)

Precautions

• Requires the use of a 96-well UV microplate for detection.
• Sample processing must be performed on ice and assays completed on the same day.
• For cell samples, it is recommended to use 3–5×10⁶ cells; use Assay Buffer for grinding or sonication during extraction, avoiding the use of cell lysis buffer.
• Prepare mixed reagents immediately before use and keep them on ice at all times.
• The assay process should be rapid; only 1–2 blank tubes need to be set up.

Storage & Shipping

Storage Conditions: Store protected from light at -20°C. Please refer to the manual for specific storage requirements of individual components. The shelf life is 12 months (from the date of shipment).

Shipping Conditions: Shipped with blue ice packs.

Applications

This research tool is suitable for scientific research in fields such as cell metabolism, oxidative stress, immunomodulation, and environmental toxicology. It is not intended for human use or clinical diagnosis.

Warning: This product is for research use only. Prior to use, ensure that it does not infringe upon any patents or licensing regulations. The manufacturer is not liable for any patent infringements or other compliance issues arising from the use of this product.

Also Known As: Glutathione Peroxidase Assay Kit; GSH-Px Activity Assay Kit; Glutathione Peroxidase Activity Assay Kit

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.