CheKine™ 胰蛋白酶活性检测试剂盒（微量法）

CheKine™ 胰蛋白酶活性检测试剂盒（微量法）

产品基础信息

货号：KTB2320

产品分类：核心产品（细胞分析）

检测类型：氨基酸代谢

样本类型：动植物组织生物样本

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ

核心特点与优势

• 精准测定动植物组织样本的胰蛋白酶活性
• 提供标准化样品制备流程及结果计算方案

检测原理

胰蛋白酶催化水解TAME酯键，释放的游离羧基与NaOH中和，导致溶液pH值降低。以苯酚红为指示剂，通过测定555 nm处吸光度变化，快速获取酶活性数据。

操作与保存

保存建议：4℃避光保存，有效期6个月

运输条件：冰袋运输（蓝冰）

注意事项：

• 推荐使用新鲜样本，-80℃可保存数周
• 解冻需控制温度与时间（室温解冻需4小时内完成）
• 实验前确保所有组分及设备温度达标

订购信息

规格1：48 T/48 S | 价格：598元 | 货期：现货（次日发货）

规格2：96 T/96 S | 价格：998元 | 货期：现货（次日发货）

法律声明

警告：本产品仅限研究使用，不适用于人类或临床诊断。使用需遵守相关专利及法规，对可能产生的侵权或违规行为不承担责任。

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

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CheKine™ Trypsin Activity Assay Kit (Micro Method)

Basic Product Information

Catalog Number:KTB2320

Product Category:Core Products (Cell Analysis)

Assay Type:Amino Acid Metabolism

Sample Type:Biological Samples from Animal and Plant Tissues

Kit Components

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ

Core Features and Advantages

• Accurate measurement of trypsin activity in animal and plant tissue samples
• Provides standardized sample preparation procedures and result calculation schemes

Assay Principle

Trypsin catalyzes the hydrolysis of TAME ester bonds, releasing free carboxyl groups that neutralize with sodium hydroxide, leading to a decrease in solution pH. Using phenol red as an indicator, enzyme activity data is rapidly obtained by measuring absorbance changes at 555 nm.

Operation and Storage

Storage Recommendation:Store at 4°C protected from light, valid for 6 months

Shipping Conditions:Shipped with ice packs (blue ice)

Precautions:

• Fresh samples are recommended; samples can be stored at -80°C for several weeks
• Thawing should be controlled in terms of temperature and time (thawing at room temperature should be completed within 4 hours)
• Ensure all components and equipment reach the required temperature before the experiment

Ordering Information

Specification 1:48 T/48 S | Price:598 RMB | Lead Time:In stock (shipped the next day)

Specification 2:96 T/96 S | Price:998 RMB | Lead Time:In stock (shipped the next day)

Legal Disclaimer

Warning:This product is for research use only and is not intended for human or clinical diagnosis. Use must comply with relevant patents and regulations, and we are not responsible for any infringement or violation that may arise.

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Note: The above information may change due to product optimization and upgrades. For specific information, please consult Yakeyin Company.