α-L-Af试剂盒，用于样本中α-L-阿拉伯呋喃糖苷酶活性检

CheKine™ 微量法 α-L-阿拉伯呋喃糖苷酶（α-L-Af）活性检测试剂盒

货号: KTB4053-E
产品分类: 核心产品 · 细胞代谢检测
样本兼容性: 植物组织、细菌、真菌及液体样本

产品概述

CheKine™ α-L-阿拉伯呋喃糖苷酶（α-L-Af）活性检测试剂盒（微量法）是一款基于比色法原理开发的高灵敏度检测工具，用于精准定量多种生物样本中的 α-L-Af 酶活性。该酶在细胞壁多糖降解、果实成熟软化等代谢研究中具有关键作用，尤其适用于植物生理学、微生物学及食品科学研究领域。

检测原理

本试剂盒通过 α-L-Af 酶催化底物对硝基酚阿拉伯呋喃糖苷（pNP-α-L-Arabinofuranoside）水解，生成在 400 nm 处具有特征吸收峰的对硝`基`苯`酚（pNP）。通过动态监测吸光度变化速率，可准确计算出酶活性单位，实现对生物样本中 α-L-Af 活性的可靠定量。

组分内容（每盒）

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Standard

技术优势

• 广泛的样本适用性：经验证可用于植物组织、细菌、真菌及液体样本，提供灵活的实验设计空间；
• 操作流程优化：试剂预配制成型，减少操作步骤，提高实验重复性与效率；
• 高灵敏度与稳定性：微量法设计，降低样本消耗，同时保证检测结果的准确性与线性范围。

保存与运输

保存条件：-20℃ 避光保存，有效期 6 个月
运输方式：蓝冰冰袋运输，确保试剂稳定性

注意事项

• 推荐使用新鲜样本。如需保存，请于 -80℃ 冻存，避免反复冻融；
• 解冻后的样本应在 4 小时内完成检测，以保证酶活性不受影响；
• 实验前请确保所有试剂及设备处于适宜温度，以保证反应一致性。

规格与定价

警告： 本产品仅用于科研用途，不适用于人类或临床诊断。使用者应负责确保应用方式不侵犯任何专利或许可协议。

如需了解更多技术细节或应用支持，请联系我们获取完整产品手册与引用文献列表。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro α-L-Arabinofuranosidase (α-L-Af) Activity Assay Kit

Catalog Number: KTB4053-E
Product Category: Core Products · Cellular Metabolism Assays
Sample Compatibility: Plant tissues, bacteria, fungi, and liquid samples

Product Overview

The CheKine™ Micro α-L-Arabinofuranosidase (α-L-Af) Activity Assay Kit is a highly sensitive colorimetric tool designed for the precise quantification of α-L-Af enzyme activity in a variety of biological samples. This enzyme plays a key role in metabolic studies such as cell wall polysaccharide degradation and fruit ripening/softening, making it particularly suitable for research in plant physiology, microbiology, and food science.

Assay Principle

This kit utilizes the hydrolysis of the substrate p-nitrophenyl α-L-arabinofuranoside (pNP-α-L-Arabinofuranoside) by α-L-Af to generate p-nitrophenol (pNP), which exhibits a characteristic absorption peak at 400 nm. By dynamically monitoring the rate of absorbance change, the enzyme activity units can be accurately calculated, enabling reliable quantification of α-L-Af activity in biological samples.

Kit Components (per kit)

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Standard

Technical Advantages

• Broad Sample Applicability: Validated for use with plant tissues, bacteria, fungi, and liquid samples, offering flexibility in experimental design;
• Optimized Workflow: Pre-formulated reagents reduce handling steps and improve experimental reproducibility and efficiency;
• High Sensitivity and Stability: Microplate method reduces sample consumption while ensuring accuracy and a broad linear detection range.

Storage and Shipping

Storage Conditions: Store protected from light at -20°C; stable for 6 months
Shipping Method: Shipped with blue ice packs to ensure reagent stability

Precautions

• Fresh samples are recommended. If storage is necessary, freeze at -80°C and avoid repeated freeze-thaw cycles;
• Thawed samples should be assayed within 4 hours to prevent loss of enzyme activity;
• Ensure all reagents and equipment are equilibrated to the appropriate temperature prior to the assay to maintain reaction consistency.

Specifications and Pricing

Warning: This product is for research use only. It is not intended for human or clinical diagnostic use. The user is responsible for ensuring that application does not infringe any patents or licensing agreements.

For detailed technical information or application support, please contact us for the complete product manual and a list of supporting publications.

Note: The above information is subject to change due to product optimization and updates. Please consult with Aokain Company for the most current details.