离子结合型果胶含量检测试剂盒，用于对样本中ISP含量检测，I

离子结合型果胶（ISP）含量检测试剂盒（微量法）

货号：KTB3016-C

产品概述：

CheKine™ 离子结合型果胶（ISP）含量检测试剂盒（微量法）是一款专为植物组织样本中离子结合型果胶（ISP）含量精准定量而设计的核心产品。本试剂盒采用成熟的咔`唑比色法原理，通过特异性螯合剂酸溶液提取ISP，并利用半乳糖醛酸与咔`唑在硫酸环境中生成特定显色复合物的特性，在530 nm波长下进行高特异性吸光度检测，实现对ISP含量的可靠分析。

产品特点与优势

• 广泛验证的样本兼容性：经过多种植物组织样本验证，确保结果可靠性。
• 操作流程简便高效：优化后的实验步骤减少繁琐操作，提升检测效率。
• 高特异性检测：针对性提取离子结合型果胶，避免其他果胶形式干扰。

试剂盒组分

• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

适用样本类型

植物组织（如果蔬、谷物等新鲜或低温保存样本）

保存条件与运输

保存条件：4℃避光保存，有效期6个月。

运输条件：冰袋（蓝冰）低温运输，确保试剂稳定性。

注意事项

• 推荐使用新鲜样本，若需保存请置于-80℃环境（可存放数周）。解冻过程需严格控制温度与时间，室温解冻应在4小时内完成。
• 实验前请确保所有试剂及设备处于适宜温度，以保证检测结果的准确性。

技术背景

果胶作为植物细胞初生壁和中胶层的关键组分，其结构复杂，包含离子结合型果胶（ISP）、水溶性果胶（WSP）和共价结合型果胶（CSP）等形式。ISP通过Ca²⁺桥、离子键及氢键等与其他细胞壁成分交联，对植物组织的质地和食品加工特性具有重要影响。本试剂盒通过特异性螯合提取结合咔`唑比色法，实现对ISP的精准定量。

规格与价格

规格	价格（元）	货期
48 T/48 S	478	现货（次日发货）
96 T/96 S	898	现货（次日发货）

警告：本产品仅限科学研究使用，不适用于人类或临床诊断。用户需确保应用场景不违反相关专利或许可协议，厂家不对专利侵权或违规使用承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Ion-Bound Pectin (ISP) Content Assay Kit (Micro Method)

Catalog Number:KTB3016-C

Product Overview:

CheKine™ Ion-Bound Pectin (ISP) Content Assay Kit (Micro Method) is a core product specifically designed for the accurate quantification of ion-bound pectin (ISP) content in plant tissue samples. This kit employs the well-established carbazole colorimetric principle. It utilizes a specific chelator acid solution to extract ISP and exploits the property of galacturonic acid to form a specific chromogenic complex with carbazole in a sulfuric acid environment. High-specificity absorbance detection at 530 nm enables reliable analysis of ISP content.

Features and Advantages

• Extensively Validated Sample Compatibility: Validated with various plant tissue samples to ensure result reliability.
• Simple and Efficient Operation: Optimized experimental procedure reduces tedious steps and improves detection efficiency.
• High Specificity Detection: Specifically targets the extraction of ion-bound pectin, avoiding interference from other pectin forms.

Kit Components

• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

Applicable Sample Types

Plant tissues (e.g., fresh or cryopreserved samples of fruits, vegetables, grains, etc.)

Storage Conditions and Shipping

Storage Conditions: Store protected from light at 4°C; stable for 6 months.

Shipping Conditions: Shipped with ice packs (blue ice) at low temperature to ensure reagent stability.

Precautions

• Fresh samples are recommended. If storage is necessary, keep at -80°C (can be stored for several weeks). The thawing process must strictly control temperature and time; thawing at room temperature should be completed within 4 hours.
• Ensure all reagents and equipment are at appropriate temperatures before the experiment to guarantee the accuracy of detection results.

Technical Background

Pectin is a key component of the plant cell primary wall and middle lamella, with a complex structure including forms such as ion-bound pectin (ISP), water-soluble pectin (WSP), and covalently-bound pectin (CSP). ISP crosslinks with other cell wall components via Ca²⁺ bridges, ionic bonds, and hydrogen bonds, significantly influencing the texture of plant tissues and characteristics in food processing. This kit achieves precise quantification of ISP through specific chelation extraction combined with the carbazole colorimetric method.

Specifications and Pricing

Specification	Price (¥)	Lead Time
48 T/48 S	478	In Stock (Ships Next Day)
96 T/96 S	898	In Stock (Ships Next Day)

Warning: This product is for research use only, not intended for human or clinical diagnostic use. Users must ensure the application does not infringe relevant patents or licensing agreements. The manufacturer assumes no liability for patent infringement or misuse.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Acquin Biotech for specific details.