ACC试剂盒，用于样本中乙酰辅酶A羧化酶活性检测，ACC A

CheKine™ 乙酰辅酶A羧化酶（ACC）活性检测试剂盒（微量法）

货号：KTB1261-E
产品类型：细胞代谢检测试剂盒
样本兼容性：动植物组织、细菌和细胞、血清（浆）及其他液体样本

产品概述

CheKine™ Micro Acetyl CoA Carboxylase (ACC) Activity Assay Kit 采用比色法原理，通过检测ACC催化反应中生成的无机磷含量，精准测定各类生物样本中乙酰辅酶A羧化酶的活性。ACC是脂肪酸合成及代谢调控中的关键限速酶，其活性直接影响脂质代谢通路的速率与方向。

核心组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Reagent Ⅴ
• Reagent VI
• Standard

技术优势

• 广泛的样本适用性：经验证适用于多种样本类型，包括动植物组织、微生物、细胞提取物及液体样本
• 操作流程精简：优化后的反应体系显色稳定，无需复杂预处理，减少操作误差
• 高兼容性与稳定性：试剂盒在-20℃避光条件下可稳定保存6个月，运输全程蓝冰保护

实验注意事项

• 推荐使用新鲜样本。若需保存，请于-80℃条件下冻存，并注意避免反复冻融
• 解冻过程需严格控制时间与温度，室温解冻应在4小时内完成检测
• 实验前请确保所有试剂恢复到适宜温度，以保证反应体系的一致性

规格与供货信息

应用背景

乙酰辅酶A羧化酶（ACC）是脂肪酸生物合成途径中的第一个关键酶，催化乙酰辅酶A转化为丙二酰辅酶A。该过程不仅影响油脂积累、能量代谢，也与胰岛素敏感性、肥胖等生理病理过程密切相关。本试剂盒为脂代谢研究、农业育种、微生物合成生物学等领域提供了可靠的酶活检测工具。

使用提示：本产品仅用于科学研究，不适用于临床诊断或人体应用。使用前请阅读相关文献并确保符合实验室操作规范。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Acetyl-CoA Carboxylase (ACC) Activity Assay Kit

Catalog Number: KTB1261-E
Product Type: Cell Metabolism Assay Kit
Sample Compatibility: Animal and plant tissues, bacteria and cells, serum (plasma), and other liquid samples

Product Overview

The CheKine™ Micro Acetyl-CoA Carboxylase (ACC) Activity Assay Kit utilizes a colorimetric method to accurately determine acetyl-CoA carboxylase activity in various biological samples by measuring the inorganic phosphate content generated in the ACC-catalyzed reaction. ACC is a key rate-limiting enzyme in fatty acid synthesis and metabolic regulation, and its activity directly influences the rate and direction of lipid metabolic pathways.

Core Components

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
• Reagent VI
• Standard

Technical Advantages

• Broad Sample Applicability: Validated for use with various sample types, including animal and plant tissues, microorganisms, cell lysates, and liquid samples.
• Streamlined Workflow: The optimized reaction system provides stable color development, requires no complex pretreatment, and minimizes operational errors.
• High Compatibility and Stability: The kit can be stored stably for 6 months at -20°C protected from light and is shipped with blue ice throughout transit.

Experimental Considerations

• Fresh samples are recommended. If storage is necessary, freeze at -80°C and avoid repeated freeze-thaw cycles.
• The thawing process must be strictly controlled for time and temperature; samples thawed at room temperature should be assayed within 4 hours.
• Ensure all reagents are equilibrated to the appropriate temperature prior to the experiment to maintain reaction consistency.

Specifications and Supply Information

Application Background

Acetyl-CoA carboxylase (ACC) is the first key enzyme in the fatty acid biosynthesis pathway, catalyzing the conversion of acetyl-CoA to malonyl-CoA. This process not only affects lipid accumulation and energy metabolism but is also closely related to physiological and pathological processes such as insulin sensitivity and obesity. This kit provides a reliable tool for enzyme activity detection in lipid metabolism research, agricultural breeding, microbial synthetic biology, and other fields.

Usage Note: This product is intended for research use only and is not suitable for clinical diagnosis or human application. Please review relevant literature and ensure compliance with laboratory operating procedures prior to use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakonin Company.