糖原合酶试剂盒，用于样本中GS活性检测，微量法，GCS Ac

糖原合酶(GCS)活性检测试剂盒 (微量法)

货号：KTB1341-S

产品概述

本产品是一款基于比色法原理的微量检测试剂盒，专为精准测定动物组织、细胞或细菌样本中的糖原合酶（Glycogen Synthase, GCS）活性而设计。GCS是糖原合成通路中的限速酶，也是胰岛素作用的关键靶点，其在维持机体糖代谢平衡与血糖稳定中扮演着核心角色。本试剂盒提供了优化的反应体系与详细的方案，适用于基础科学研究。

检测原理

试剂盒基于酶偶联反应原理。GCS催化底物UDPG和葡萄糖残基生成糖原和UDP；随后，丙`酮酸激酶与乳酸脱氢酶依次催化NADH氧化生成NAD+。通过在340 nm波长下监测NADH的下降速率，即可准确计算出GCS的酶活性。

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V

产品特点与优势

• 可适用于多种样本类型，包括动物组织、细胞提取物及细菌样本；
• 采用微量法设计，节省样品与试剂；
• 提供完整的样品制备指南与活性计算公式，便于实验标准化与数据重复；
• 适用于96孔UV微孔板高通量检测模式。

规格与价格

规格	价格（元）	货期
48 T/48 S	1758	现货（次日发货）
96 T/96 S	2639	现货（次日发货）

注意事项

• 实验需使用96孔UV微孔板；
• 严禁混合使用不同批次或不同厂家的试剂组分；
• 样本制备及处理过程需全程在冰上操作，且匀浆液应避免反复冻融；
• 建议在当日完成酶活性测定，以保证结果准确性；
• 实验前请确保所有试剂及设备均已达到适宜温度。

保存与运输

建议收到货后避光保存于-20°C，有效期6个月。具体各组分保存条件请详见说明书。本品采用蓝冰冰袋运输，以保证试剂稳定性。

警告

本产品仅限用于科学研究，不适用于人类或临床诊断。使用者应自行负责确保应用方式不侵犯任何专利或许可协议。本公司不对因使用本产品所可能导致的专利侵权或其他违规行为承担任何责任。

别名：Micro Glycogen synthase(GCS) Activity Assay Kit, 糖原合酶, 糖原合酶活性检测, 糖原合酶活性检测试剂盒, GCS

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Glycogen Synthase (GCS) Activity Assay Kit (Micro Method)

Catalog Number: KTB1341-S

Product Overview

This product is a microassay kit based on the colorimetric method, specifically designed for the accurate determination of Glycogen Synthase (GCS) activity in animal tissues, cells, or bacterial samples. GCS is the rate-limiting enzyme in the glycogen synthesis pathway and a key target of insulin action, playing a central role in maintaining glucose metabolic balance and blood sugar homeostasis. This kit provides an optimized reaction system and a detailed protocol, suitable for basic scientific research.

Assay Principle

The kit is based on the principle of an enzyme-coupled reaction. GCS catalyzes the substrate UDPG and glucose residues to produce glycogen and UDP; subsequently, pyruvate kinase and lactate dehydrogenase sequentially catalyze the oxidation of NADH to NAD+. By monitoring the rate of NADH decrease at a wavelength of 340 nm, the enzymatic activity of GCS can be accurately calculated.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V

Features and Advantages

• Applicable to various sample types, including animal tissues, cell lysates, and bacterial samples;
• Designed with a micro method, saving samples and reagents;
• Provides complete sample preparation guidelines and activity calculation formulas for experimental standardization and data reproducibility;
• Suitable for high-throughput detection using 96-well UV microplates.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
48 T/48 S	1758	In Stock (Ships Next Day)
96 T/96 S	2639	In Stock (Ships Next Day)

Precautions

• Experiments require the use of 96-well UV microplates;
• Do not mix reagents from different batches or different manufacturers;
• Sample preparation and handling must be performed entirely on ice, and homogenates should avoid repeated freeze-thaw cycles;
• It is recommended to complete the enzyme activity assay on the same day to ensure result accuracy;
• Before the experiment, ensure all reagents and equipment have reached the appropriate temperature.

Storage and Shipping

Upon receipt, it is recommended to store protected from light at -20°C; the product is valid for 6 months. For specific storage conditions of each component, please refer to the manual. This product is shipped with blue ice packs to ensure reagent stability.

Warning

This product is for research use only, not intended for human or clinical diagnostic use. Users are responsible for ensuring that their application does not infringe any patents or licensing agreements. The company assumes no liability for any potential patent infringement or other violations resulting from the use of this product.

Also Known As: Micro Glycogen synthase(GCS) Activity Assay Kit, Glycogen Synthase, Glycogen Synthase Activity Assay, Glycogen Synthase Activity Assay Kit, GCS

Note: The above information may change due to product optimization and upgrades. For specific details, please consult Yakin Company.