Phospho-IRS1 (Tyr632) Rabbit P

olyclonal Antibody 抗体，orb317637，Biorbyt

价格￥3159 - 5850

品牌Biorbyt
货号orb317637
产地英国
更新日期2026年07月22日

应用范围FC, ICC, IF, IHC-Fr, IHC-P
宿主Rabbit
适应物种Human, Mouse, Rat

13 年钻石会员

相关产品推荐更多 >

万千商家帮你免费找货

0 人在求购买到急需产品

详细信息
文献和实验
技术资料

抗体名：
Phospho-IRS1 (Tyr632) Rabbit Polyclonal Antibody 抗体
抗体英文名：
Phospho-IRS1 (Tyr632) Rabbit Polyclonal Antibody
靶点：
IRS1
浓度：
1mg/ml
应用范围：
FC, ICC, IF, IHC-Fr, IHC-P
宿主：
Rabbit
适应物种：
Human, Mouse, Rat
保质期：
12 months from date of receipt.
抗原来源：
详询
目录编号：
orb317637
级别：
科研
库存：
99
供应商：
Biorbyt
标记物：
Unconjugated
克隆性：
Polyclonal
保存条件：
详询
形态：
Liquid
亚型：
IgG
免疫原：
详询
规格：
50 ul/100 ul/200 ul

规格：	50 ul	产品价格：	￥3159.0
规格：	100 ul	产品价格：	￥4914.0
规格：	200 ul	产品价格：	￥5850.0

类别: Antibodies

产品子类型: Primary Antibody

描述: Phospho-IRS1 (Tyr632) Rabbit Polyclonal Antibody

别名: HIRS-1; G972R; IRS-1; IRS1IRM; IRS1_HUMAN; IRS1; IRS1_MOUSE; IRS1_RAT; pp185; insulin receptor substrate 1; IRS1 (phospho-Y632); p-IRS1; phospho-IRS1; IRS1 (phospho-Tyr632)

应用稀释比例: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

免疫原: KLH conjugated Synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Tyr632 GD(p-Y)MP

分子量: 132 kDa

预测反应性: Bovine, Canine, Guinea pig, Porcine, Rabbit, Sheep

防腐剂: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.

纯化方法: Affinity purified by Protein A

研究领域: Signal Transduction

UniProt ID: P35568

保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

备注: For research use only.

A549 cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-IRS1 (Tyr632)) polyclonal Antibody, Unconjugated (orb317637) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-phospho-IRS1 (Tyr632) antibody (orb317637), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Fixative-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-IRS1 (Tyr632)) Polyclonal Antibody, Unconjugated (orb317637) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Fixative-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-IRS1 (Tyr632)) Polyclonal Antibody, Unconjugated (orb317637) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

文献和实验

大家都在搜

大家都在搜