土壤荧光素二乙酸酯水解酶活性检测试剂盒，用于对样本中S-FD

产品概述

货号：KTB4049-C
产品名称：土壤荧光素二乙酸酯水解酶活性检测试剂盒（Micro Soil-Fluorescein Diacetate (S-FDA) Hydrolase Activity Assay Kit）
产品分类：核心产品
应用中心：细胞分析 - 细胞代谢

应用背景

土壤荧光素二乙酸酯水解酶（S-FDA）活性是评估土壤微生物活性、土壤质量变化及生态系统中有机质转化的重要生物学指标。本试剂盒采用微量比色法，适用于土壤样本中S-FDA水解酶活性的精准检测，为环境科学研究与农业生态监测提供可靠工具。

检测原理

FDA作为一种无色底物，可在土壤酶催化下发生水解反应，并进一步脱水生成稳定且具强荧光特性的终产物——荧光素。该产物在490 nm波长处具有特征吸收峰，通过测定该波长下的吸光度变化，即可准确计算出S-FDA水解酶的活性水平。

产品特点与优势

• 经验证适用于多种土壤样本类型，兼容性强
• 操作流程简便，检测结果稳定可靠
• 提供48T与96T两种规格，满足不同通量需求
• 组分明确，包含Reagent I、Reagent Ⅱ及Standard

技术参数

检测方法：比色法（微量法）
样本类型：土壤样本
检测指标：土壤荧光素二乙酸酯水解酶（S-FDA）活性
别名：土壤荧光素二乙酸酯水解酶（S-FDA）

规格与订购信息

保存与运输

保存条件：4℃避光保存，有效期6个月
运输方式：冰袋运输（蓝冰）

注意事项

• 推荐使用新鲜土壤样本，若需保存，请于-80℃条件下存放，数周内完成检测
• 解冻样本时应严格控制温度与时间，室温解冻需在4小时内完成
• 实验前请确保所有试剂及设备处于适宜温度，以保证检测准确性

重要声明

本产品仅限于科研用途，不适用于人类或临床诊断。使用者应自行确保应用方式不侵犯任何专利权或许可证规定，本公司不对因使用本产品所导致的专利侵权或其他违规行为承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Catalog Number: KTB4049-C
Product Name: Soil-Fluorescein Diacetate (S-FDA) Hydrolase Activity Assay Kit (Micro Method)
Product Classification: Core Product
Application Center: Cell Analysis - Cell Metabolism

Application Background

Soil-Fluorescein Diacetate (S-FDA) hydrolase activity is a crucial biological indicator for assessing soil microbial activity, soil quality changes, and organic matter transformation in ecosystems. This kit employs a micro-colorimetric method, suitable for the accurate detection of S-FDA hydrolase activity in soil samples, providing a reliable tool for environmental science research and agricultural ecological monitoring.

Detection Principle

FDA, as a colorless substrate, undergoes hydrolysis catalyzed by soil enzymes, and is further dehydrated to generate a stable end product with strong fluorescence—fluorescein. This product exhibits a characteristic absorption peak at 490 nm. By measuring the absorbance change at this wavelength, the activity level of S-FDA hydrolase can be accurately calculated.

Features and Advantages

• Validated for various soil sample types with strong compatibility
• Simple operational procedure with stable and reliable results
• Available in 48T and 96T specifications to meet different throughput needs
• Well-defined components including Reagent I, Reagent II, and Standard

Technical Parameters

Assay Method: Colorimetric method (Micro method)
Sample Type: Soil samples
Detection Target: Soil-Fluorescein Diacetate (S-FDA) Hydrolase Activity
Alias: Soil-Fluorescein Diacetate (S-FDA)

Specifications and Ordering Information

Storage and Shipping

Storage Conditions: Store protected from light at 4°C, valid for 6 months.
Shipping Method: Shipped with ice packs (blue ice).

Precautions

• Fresh soil samples are recommended. If storage is necessary, store at -80°C and complete the assay within a few weeks.
• Thawing samples requires strict control of temperature and time; thawing at room temperature should be completed within 4 hours.
• Ensure all reagents and equipment are at appropriate temperatures before the experiment to guarantee assay accuracy.

Important Statement

This product is for research use only, not intended for human or clinical diagnostic use. Users are responsible for ensuring that their application does not infringe any patent rights or license regulations. The company shall not be held liable for any patent infringement or other violations resulting from the use of this product.

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Yacoin Corporation.