丙`酮酸脱氢酶活性检测试剂盒，用于对样本中PDH活性检测，P

丙`酮酸脱氢酶（PDH）活性检测试剂盒（微量法）

货号：KTB1270-C
产品类型：细胞代谢检测试剂盒
样本类型：适用于动植物组织、细胞和细菌样本
检测方法：比色法（605 nm）

产品概述

CheKine™ 丙`酮酸脱氢酶（PDH）活性检测试剂盒（微量法）是一款专为科研用户设计的核心产品，用于准确、便捷地定量检测多种生物样本中PDH的酶活性。PDH作为连接糖酵解与三羧酸循环的关键限速酶，在细胞能量代谢研究中具有重要作用。本试剂盒采用优化的微量法检测体系，操作流程清晰，重复性良好，适合高水平研究使用。

检测原理

PDH催化丙`酮酸脱氢，还原2,6-二氯靛酚（2,6-DCPIP），通过在605 nm波长下监测2,6-DCPIP吸光度的下降速率，可准确计算出PDH酶活性水平。

试剂盒组分

• Extraction BufferⅠ
• Extraction BufferⅡ
• ReagentⅠ
• ReagentⅡ
• ReagentⅢ

特点与优势

• 可广泛用于动植物组织、细胞提取物及细菌样本的PDH活性检测；
• 提供完整的样品处理方案与活性计算公式，协助用户规范实验操作；
• 试剂组分经优化配比，灵敏度高，适应微量检测需求。

保存与运输

保存条件：-20℃避光保存，有效期6个月
运输方式：冰袋（蓝冰）低温运输

注意事项

• 建议使用新鲜样本进行检测，如需保存，请在-80℃条件下存放，避免反复冻融；
• 样本解冻应在室温下快速完成，并在4小时内完成测定；
• 实验前请确保所有试剂及设备处于适宜温度，以保证检测结果的准确性。

规格与货期

规格1：48 T/48 S   |   价格：626元   |   货期：现货，次日发货
规格2：96 T/96 S   |   价格：1016元   |   货期：现货，次日发货

警告：本品仅供科研使用，不适用于临床诊断或人体使用。用户应确保应用符合相关法律法规及知识产权要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Pyruvate Dehydrogenase (PDH) Activity Assay Kit (Micro-Scale Method)

Catalog Number: KTB1270-C
Product Type: Cell Metabolism Assay Kit
Sample Types: Suitable for animal/plant tissues, cells, and bacterial samples
Assay Method: Colorimetric method (605 nm)

Overview

The CheKine™ Pyruvate Dehydrogenase (PDH) Activity Assay Kit (Micro-Scale Method) is a core product specifically designed for research users to accurately and conveniently quantify PDH enzyme activity in a variety of biological samples. As a key rate-limiting enzyme connecting glycolysis and the tricarboxylic acid (TCA) cycle, PDH plays a crucial role in cellular energy metabolism research. This kit employs an optimized micro-scale assay system with a clear operational procedure and excellent reproducibility, making it suitable for high-level research applications.

Assay Principle

PDH catalyzes the dehydrogenation of pyruvate, reducing 2,6-dichloroindophenol (2,6-DCPIP). The enzyme activity level of PDH can be accurately calculated by monitoring the rate of decrease in absorbance of 2,6-DCPIP at 605 nm.

Kit Components

• Extraction BufferⅠ
• Extraction BufferⅡ
• ReagentⅠ
• ReagentⅡ
• ReagentⅢ

Features & Advantages

• Broadly applicable for PDH activity detection in animal/plant tissues, cell lysates, and bacterial samples;
• Provides complete sample handling protocols and activity calculation formulas to assist users in standardizing experimental operations;
• Reagent components are optimally formulated for high sensitivity, meeting the requirements of micro-scale assays.

Storage & Shipping

Storage Conditions: Store protected from light at -20°C; stable for 6 months.
Shipping Conditions: Shipped with ice packs (blue ice) at low temperatures.

Precautions

• Fresh samples are recommended for assay. If storage is necessary, store at -80°C and avoid repeated freeze-thaw cycles;
• Thaw samples quickly at room temperature and complete the assay within 4 hours;
• Ensure all reagents and equipment are at appropriate temperatures prior to the experiment to guarantee accurate results.

Specifications & Availability

Specification 1: 48 T/48 S   |   Price: ¥626   |   Availability: In stock, ships next day
Specification 2: 96 T/96 S   |   Price: ¥1016   |   Availability: In stock, ships next day

Warning: This product is for research use only. Not intended for clinical diagnosis or human use. Users must ensure applications comply with relevant laws, regulations, and intellectual property requirements.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Abbkine for specific details.