一氧化氮含量检测试剂盒，用于对样本中NO含量检测，NO As

一氧化氮含量检测试剂盒 (Micro Nitric Oxide (NO) Assay Kit)

货号: KTB1400-C

产品中心: 细胞分析 | 产品分类: 核心产品

产品概述

本产品是一种高性能的比色法检测试剂盒，专门用于精准测量各类生物样本中一氧化氮（NO）的含量。一氧化氮作为关键的信号分子和自由基，广泛参与神经传递、免疫调节、细胞凋亡及血管舒张等多种生理与病理过程。本试剂盒通过优化的检测方案，为研究者提供快速、可靠的高通量分析工具。

检测原理

试剂盒采用经典的格里斯(Griess)反应原理，并引入高效还原剂VCl₃，显著缩短了将硝酸盐（NO₃⁻）还原为亚硝酸盐（NO₂⁻）所需的时间。还原产生的亚硝酸盐与 Griess 试剂发生显色反应，通过比色法在特定波长下测定吸光度值，从而准确计算出样本中NO的含量。

特点与优势

• 广泛的样本兼容性: 适用于血清、血浆、尿液、动物组织/细胞裂解液、植物样本以及其他多种生物液体。
• 宽广的检测范围: 检测范围为1 µM 至 100 µM，能够满足绝大多数实验需求。
• 快速且高效: 优化的VCl₃还原步骤大幅缩短了反应时间，实现了快速检测；96孔板模式支持高通量自动化操作，可高效处理大量样本。
• 操作简便: 流程清晰，仅需将样本与试剂混合，经过孵育后读取光密度即可。

试剂盒组分

• NaNO₂ 标准品 (1M)
• 格里斯试剂 I (Griess Reagent I)
• 格里斯试剂 II (Griess Reagent II)
• VCl₃ 试剂 (Vanadium(III) Chloride Reagent)
• ZnSO₄ (Zinc Sulfate)

技术参数

• 检测方法: 比色法
• 检测类型: 氧化应激
• 检测指标: 一氧化氮 (NO)

注意事项

• 为确保测量结果的准确性，建议对样本进行梯度稀释，以确保最终读数落在标准曲线的线性范围内。
• 推荐使用新鲜制备的样本进行检测。若不能立即检测，样本应于-80℃条件下保存，且保存期限不宜超过一个月。

保存与运输

• 保存建议: 请于-20°C避光保存。自收货之日起，试剂盒整体有效期可达12个月。各组分具体保存条件请详见产品说明书。
• 运输条件: 全程采用冰袋（蓝冰）低温运输，以确保试剂稳定性。

规格与价格

规格	价格 (人民币)	货期
48 T/48 S	278	现货（当天发货）
96 T/96 S	426	现货（当天发货）

警告: 本产品仅用于科学研究用途，不适用于人类或临床诊断。使用者应确保其应用不侵犯任何专利或许可证，本公司对此不承担任何责任。

别名: 一氧化氮, 一氧化氮（NO）检测, 一氧化氮（NO）检测试剂盒, NO, Nitric Oxide

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Nitric Oxide (NO) Assay Kit

Catalog Number: KTB1400-C

Product Center: Cell Analysis | Category: Core Products

Product Overview

This product is a high-performance colorimetric assay kit specifically designed for the accurate measurement of nitric oxide (NO) levels in various biological samples. As a critical signaling molecule and free radical, nitric oxide is extensively involved in numerous physiological and pathological processes, including neurotransmission, immune regulation, apoptosis, and vasodilation. With an optimized detection protocol, this kit provides researchers with a rapid and reliable tool for high-throughput analysis.

Assay Principle

The kit employs the classic Griess reaction principle, incorporating the efficient reducing agent VCl₃ to significantly shorten the time required for the reduction of nitrate (NO₃⁻) to nitrite (NO₂⁻). The reduced nitrite reacts with the Griess reagent to produce a chromogenic product. The absorbance is measured at a specific wavelength via colorimetry, enabling accurate calculation of the NO concentration in the sample.

Features and Advantages

• Broad Sample Compatibility: Suitable for serum, plasma, urine, animal tissue/cell lysates, plant samples, and various other biological fluids.
• Wide Detection Range: Detection range from 1 µM to 100 µM, meeting the demands of most experimental requirements.
• Rapid and Efficient: The optimized VCl₃ reduction step greatly shortens reaction time, enabling fast detection; the 96-well plate format supports high-throughput automated processing for efficient handling of large sample numbers.
• Simple Operation: Clear procedure involves only mixing the sample with reagents, followed by incubation and optical density reading.

Kit Components

• NaNO₂ Standard (1M)
• Griess Reagent I
• Griess Reagent II
• VCl₃ Reagent (Vanadium(III) Chloride Reagent)
• ZnSO₄ (Zinc Sulfate)

Technical Parameters

• Assay Method: Colorimetric
• Assay Type: Oxidative Stress
• Target: Nitric Oxide (NO)

Precautions

• To ensure measurement accuracy, it is recommended to perform gradient dilution of samples to ensure final readings fall within the linear range of the standard curve.
• Freshly prepared samples are recommended for detection. If immediate testing is not possible, samples should be stored at -80°C for no longer than one month.

Storage and Shipping

• Storage Recommendation: Store at -20°C protected from light. The overall kit is stable for 12 months from the date of receipt. Please refer to the product manual for specific storage conditions of individual components.
• Shipping Conditions: Shipped with ice packs (blue ice) to maintain a low temperature throughout transit, ensuring reagent stability.

Specifications and Pricing

Specification	Price (RMB)	Lead Time
48 T/48 S	278	In Stock (Ships Same Day)
96 T/96 S	426	In Stock (Ships Same Day)

Warning: This product is for research use only. It is not intended for human or clinical diagnostic use. The user is responsible for ensuring that its application does not infringe any patents or licenses. The company assumes no liability for such matters.

Also Known As: Nitric Oxide, Nitric Oxide (NO) Detection, Nitric Oxide (NO) Detection Kit, NO, Nitric Oxide

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.