ATP柠檬酸裂解酶活性检测试剂盒，用于对样本中ACL活性检测

ATP柠檬酸裂解酶(ACL)活性检测试剂盒 | 微量法 | 货号：KTB1252-C

CheKine™ Micro ATP Citrate Lyase (ACL) Activity Assay Kit是一款专业用于定量检测细胞、组织及生物液体样本中ATP柠檬酸裂解酶（ACL）活性的核心工具。该试剂盒采用经典的比色法原理，适用于多种样本类型，操作流程精简，结果稳定可靠。

产品概述

本试剂盒设计用于科研领域中对细胞代谢关键酶——ATP柠檬酸裂解酶的活性分析。ACL是催化胞质中柠檬酸转化为乙酰辅酶A的核心酶，在脂肪酸合成、胆固醇代谢及蛋白质乙酰化修饰等生物过程中起着枢纽作用。

检测原理

在ATP和辅酶A存在下，ACL催化柠檬酸裂解生成乙酰辅酶A、草酰乙酸、ADP和磷酸盐；草酰乙酸在苹果酸脱氢酶的作用下与NADH反应，生成苹果酸和NAD+，通过在340 nm处监测NADH的吸光度下降，即可计算出ACL的酶活性。

试剂盒组成

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ

特点与优势

• 广泛验证的样本兼容性：适用于动植物组织、细菌、细胞以及血清（浆）等多种样本；
• 操作流程简便，检测过程高效，适合中等至高通量实验需求。

样本处理建议

推荐使用新鲜样本进行检测。如不能立即实验，样本可在-80°C条件下保存数周。解冻过程需严格控制温度与时间，如在室温解冻，应在4小时内完成。

保存与运输

本品需-20℃避光保存，有效期为6个月。运输过程中采用蓝冰冰袋保温，以保障试剂稳定性。

规格与货期

规格	价格（元）	货期
48 T/48 S	1426	现货，次日发货
96 T/96 S	2378	现货，次日发货

注意事项

实验前请确保所有试剂及设备处于适宜温度。本产品仅限科研使用，不应用于人类或临床诊断。使用前请参阅相关文献并确认不侵犯任何专利权或许可协议。

别名：ATP柠檬酸裂解酶（ACL）活性检测盒 | ACL Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro ATP Citrate Lyase (ACL) Activity Assay Kit | Micro Method | Cat#: KTB1252-C

The CheKine™ Micro ATP Citrate Lyase (ACL) Activity Assay Kit is a core tool designed for the quantitative determination of ATP citrate lyase (ACL) activity in cells, tissues, and biological fluid samples. This kit employs a classic colorimetric principle, is suitable for various sample types, and features a streamlined operating procedure with stable and reliable results.

Product Overview

This kit is designed for the analysis of ATP citrate lyase activity, a key enzyme in cellular metabolism, in scientific research. ACL is a central enzyme that catalyzes the conversion of citrate to acetyl-CoA in the cytoplasm and plays a pivotal role in biological processes such as fatty acid synthesis, cholesterol metabolism, and protein acetylation modification.

Assay Principle

In the presence of ATP and coenzyme A, ACL catalyzes the cleavage of citrate to generate acetyl-CoA, oxaloacetate, ADP, and phosphate. Oxaloacetate then reacts with NADH under the action of malate dehydrogenase to produce malate and NAD+. By monitoring the decrease in absorbance of NADH at 340 nm, the enzymatic activity of ACL can be calculated.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III

Features & Advantages

• Extensively validated sample compatibility: Suitable for a variety of samples including animal and plant tissues, bacteria, cells, and serum (plasma);
• Simple operation and efficient detection process, ideal for medium to high-throughput experimental needs.

Sample Handling Recommendations

Fresh samples are recommended for assay. If immediate testing is not possible, samples can be stored at -80°C for several weeks. The thawing process must be strictly controlled for temperature and time; if thawed at room temperature, it should be completed within 4 hours.

Storage & Transportation

This product must be stored protected from light at -20°C and is stable for 6 months. During transportation, blue ice packs are used to maintain temperature and ensure reagent stability.

Specifications & Delivery

Specification	Price (¥)	Delivery Time
48 T/48 S	1426	In stock, ships next day
96 T/96 S	2378	In stock, ships next day

Precautions

Ensure all reagents and equipment are at appropriate temperatures before starting the experiment. This product is for research use only and is not intended for human or clinical diagnostic use. Please consult relevant literature and confirm non-infringement of any patent rights or license agreements prior to use.

Also known as: ATP Citrate Lyase (ACL) Activity Assay Kit | ACL Activity Assay Kit

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult YaKeYin Company.