可溶性酸性转化酶试剂盒，用于样本中S-AI活性检测，微量法，

可溶性酸性转化酶（S-AI）活性检测试剂盒（微量法）

货号：KTB2292-S

产品分类：核心产品｜细胞分析｜细胞代谢检测试剂盒

英文名称：Micro Soluble Acid Invertase (S-AI) Activity Assay Kit

产品概述

CheKine™ 可溶性酸性转化酶（S-AI）活性检测试剂盒（微量法）是一款专为植物组织样本中S-AI酶活性定量分析而设计的高灵敏度检测工具。该试剂盒采用比色法，通过测定510 nm波长下吸光度的变化，精准反映S-AI催化蔗糖分解生成还原糖的速率，适用于细胞代谢研究中蔗糖代谢途径的深入探索。

检测原理

本试剂盒基于S-AI催化蔗糖水解生成葡萄糖和果糖（还原糖），还原糖在碱性条件下与3,5-二硝基水杨酸发生显色反应，生成棕红色化合物，在510 nm处具有特征吸收峰。酶活性通过单位时间内吸光度增加值进行计算，结果可靠、重复性高。

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

规格与定价

规格	价格（元）	货期
48 T/24 S	598	现货（次日发货）
96 T/48 S	998	现货（次日发货）

保存与运输

保存建议：4℃避光保存，有效期6个月。

运输条件：冰袋运输（蓝冰）。

注意事项

• 推荐使用新鲜样本。若需保存，请于-80℃冷冻，避免反复冻融。
• 解冻样本应在室温下4小时内完成，以保证酶活性稳定。
• 实验前请确保所有试剂及设备处于适宜温度，以保证反应一致性。

背景信息

蔗糖转化酶（Invertase, Ivr）是植物蔗糖代谢中的关键酶，其中可溶性酸性转化酶（S-AI）最适pH为4.5–5.0，主要分布于液泡或细胞自由空间中，参与调节蔗糖分解与果实糖积累过程。本试剂盒为植物糖代谢研究提供了一种可靠的工具。

警告：本产品仅用于科学研究，不适用于人类或临床诊断。使用前请确认符合相关法律法规与许可要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Soluble Acid Invertase (S-AI) Activity Assay Kit

Catalog Number: KTB2292-S

Product Classification: Core Products | Cell Analysis | Cell Metabolism Assay Kits

English Name: Micro Soluble Acid Invertase (S-AI) Activity Assay Kit

Product Overview

The CheKine™ Micro Soluble Acid Invertase (S-AI) Activity Assay Kit is a highly sensitive detection tool specifically designed for the quantitative analysis of S-AI enzyme activity in plant tissue samples. This kit employs a colorimetric method to accurately reflect the rate of sucrose decomposition into reducing sugars catalyzed by S-AI by measuring the change in absorbance at 510 nm. It is suitable for in-depth exploration of sucrose metabolic pathways in cell metabolism research.

Assay Principle

This kit is based on the hydrolysis of sucrose catalyzed by S-AI to produce glucose and fructose (reducing sugars). The reducing sugars undergo a color development reaction with 3,5-dinitrosalicylic acid under alkaline conditions, generating a brownish-red compound with a characteristic absorption peak at 510 nm. Enzyme activity is calculated from the increase in absorbance per unit time, ensuring reliable and highly reproducible results.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

Specifications and Pricing

Specification	Price (¥)	Lead Time
48 T/24 S	598	In Stock (Ships Next Day)
96 T/48 S	998	In Stock (Ships Next Day)

Storage and Shipping

Storage Recommendation: Store at 4°C protected from light; valid for 6 months.

Shipping Conditions: Shipped with ice packs (blue ice).

Notes

• Fresh samples are recommended. If storage is necessary, freeze at -80°C and avoid repeated freeze-thaw cycles.
• Thawed samples should be processed within 4 hours at room temperature to ensure enzyme activity stability.
• Prior to the experiment, ensure all reagents and equipment are at appropriate temperatures to guarantee reaction consistency.

Background Information

Sucrose invertase (Ivr) is a key enzyme in plant sucrose metabolism. Soluble acid invertase (S-AI), with an optimal pH of 4.5–5.0, is primarily distributed in vacuoles or the free space of cells and is involved in regulating sucrose decomposition and fruit sugar accumulation. This kit provides a reliable tool for research on plant sugar metabolism.

Warning: This product is intended for research use only and is not suitable for human or clinical diagnostic use. Please ensure compliance with relevant laws, regulations, and licensing requirements before use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakin Company.