Ca2+/Mg2+-ATP酶试剂盒，用于样本中Ca2+/Mg

产品详情：Ca2+/Mg2+-ATP酶活性检测试剂盒（微量法）

货号：KTB1810-S

产品类型：细胞代谢检测试剂盒

检测方法：比色法

产品概述

本试剂盒是一种基于比色法原理的体外诊断试剂，设计用于定量检测血清、血浆、组织、细胞、细菌、植物及其他生物体液样本中的Ca2⁺/Mg2⁺-ATP酶活性。该酶是氧化磷酸化过程中的关键酶，广泛分布于生物膜系统，在细胞生理学中具有多种重要功能，主要催化ATP水解生成ADP和无机磷。

产品特点与优势

• 适用范围广：可测定多种生物样本中的Ca2⁺/Mg2⁺-ATP酶活性。
• 操作便捷：试剂盒提供详尽的样品制备指南和结果计算方法，简化实验流程。
• 灵敏度高：微量法设计，适合有限样本量的研究。
• 组分齐全：包含提取缓冲液、测定缓冲液及多种专用试剂，确保实验顺利进行。

试剂盒组分

• Extraction Buffer
• Assay Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
• Reagent VI
• Standard

规格与定价

注意事项

• 实验需使用96孔UV微孔板。
• 严禁混用不同批次或不同厂家的试剂组分。
• 样本处理及实验操作需在冰上进行，匀浆液应避免反复冻融，且须在当日完成酶活力测定。
• 实验前请确保所有组分及设备处于适宜温度。
• 严格使用无磷试管，避免磷污染，这是检测成功的关键。

保存与运输

保存条件：收到产品后，请避光保存于-20℃。具体各组分的存储条件请参阅说明书。自发货之日起，在推荐保存条件下，有效期为12个月。

运输条件：冰袋运输（蓝冰）。

警告

本产品仅供研究使用，不适用于人类或临床诊断。使用本产品可能涉及专利或许可证限制，对于因使用本产品而可能导致的专利侵权或其他违规行为，本公司不承担任何责任。

别名：Ca2+/Mg2+-ATPase酶, Ca2+/Mg2+-ATPase酶活性检测, Ca2+/Mg2+-ATPase酶活性检测试剂盒, ATP酶, Ca2+/Mg2+-ATPase

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Details: Ca²⁺/Mg²⁺-ATPase Activity Assay Kit (Micro Method)

Catalog Number: KTB1810-S

Product Type: Cell Metabolism Assay Kit

Assay Method: Colorimetric Method

Overview

This kit is an in vitro diagnostic reagent based on the colorimetric principle, designed for the quantitative detection of Ca²⁺/Mg²⁺-ATPase activity in samples such as serum, plasma, tissues, cells, bacteria, plants, and other biological fluids. This enzyme is a key enzyme in the oxidative phosphorylation process, widely distributed in biomembrane systems, and possesses various important functions in cell physiology, primarily catalyzing the hydrolysis of ATP to generate ADP and inorganic phosphate.

Features & Advantages

• Broad Applicability: Can measure Ca²⁺/Mg²⁺-ATPase activity in a variety of biological samples.
• Convenient Operation: The kit provides detailed sample preparation guidelines and result calculation methods to streamline the experimental procedure.
• High Sensitivity: The micro method design is suitable for studies with limited sample quantities.
• Comprehensive Components: Includes extraction buffer, assay buffer, and various specialized reagents to ensure smooth experiment progression.

Kit Components

• Extraction Buffer
• Assay Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
• Reagent VI
• Standard

Specifications & Pricing

Precautions

• The assay requires the use of a 96-well UV microplate.
• Do not mix components from different batches or manufacturers.
• Sample handling and experimental operations must be performed on ice. Homogenates should avoid repeated freeze-thaw cycles, and enzyme activity must be measured on the same day.
• Ensure all components and equipment are at appropriate temperatures before starting the experiment.
• Strictly use phosphate-free tubes to avoid phosphate contamination, which is critical for a successful assay.

Storage & Shipping

Storage Conditions: Upon receipt, store protected from light at -20°C. Refer to the manual for specific storage conditions of individual components. The product is stable for 12 months from the date of shipment when stored under recommended conditions.

Shipping Conditions: Shipped with ice packs (blue ice).

Warning

This product is for research use only. It is not intended for human or clinical diagnostic use. The use of this product may involve patent or license restrictions. The company assumes no liability for patent infringement or other violations that may result from the use of this product.

Also Known As: Ca²⁺/Mg²⁺-ATPase, Ca²⁺/Mg²⁺-ATPase Activity Assay, Ca²⁺/Mg²⁺-ATPase Activity Assay Kit, ATPase, Ca²⁺/Mg²⁺-ATPase

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Yacene Corporation.