过氧化氢酶试剂盒，用于样本中CAT活性分析，钼酸铵比色法，C

过氧化氢酶（CAT）活性检测试剂盒

货号：KTB1042-S

产品概述

本产品是一种基于钼酸铵显色法的过氧化氢酶（CAT）活性检测试剂盒，适用于动植物组织、细胞或细菌、血清（浆）等多种样本类型的CAT活性定量分析。该试剂盒通过测量H₂O₂分解反应产物，实现对CAT酶活性的高灵敏度检测，是细胞代谢研究与氧化应激机制分析的重要工具。

检测原理

过氧化氢酶（CAT）催化H₂O₂分解为H₂O和O₂，剩余H₂O₂在酸性条件下与钼酸铵反应生成黄色络合物，在405 nm波长处测定吸光度值，通过标准曲线计算CAT活性。

试剂盒组成

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III

主要参数

• 检测方法：比色法（钼酸铵显色法）
• 检测范围：3-195 mM
• 灵敏度：3 mM
• 样本类型：动植物组织、细胞/细菌、血清（浆）

技术优势

• 操作流程清晰，无需特殊仪器设备
• 显色稳定，重复性良好
• 适用于高通量样本分析

注意事项

• 建议进行预实验以优化样本量
• 推荐使用BCA法进行样本蛋白浓度均一化
• 需用户自行建立标准曲线
• 操作时需在通风橱中进行，佩戴手套等防护用具

保存与运输

• 保存条件：4℃避光保存，有效期6个月
• 运输方式：蓝冰冰链运输

安全警告

本品所含生化试剂具有一定刺激性和毒性，请严格遵守实验室生物安全规范操作。

应用背景

过氧化氢酶（CAT）是细胞抗氧化系统的关键酶之一，负责催化H₂O₂分解，防止活性氧累积造成的细胞损伤，在氧化应激相关疾病机制研究中具有重要价值。

产品规格与价格

搜索别名：过氧化氢酶、CAT、Catalase Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Catalase (CAT) Activity Assay Kit

Catalog Number: KTB1042-S

Product Overview

This product is a catalase (CAT) activity assay kit based on the ammonium molybdate chromogenic method, suitable for quantitative analysis of CAT activity in various sample types including animal and plant tissues, cells or bacteria, and serum (plasma). By measuring the products of the H₂O₂ decomposition reaction, this kit enables highly sensitive detection of CAT enzyme activity and serves as an essential tool for research in cellular metabolism and analysis of oxidative stress mechanisms.

Assay Principle

Catalase (CAT) catalyzes the decomposition of H₂O₂ into H₂O and O₂. The residual H₂O₂ reacts with ammonium molybdate under acidic conditions to form a yellow complex. The absorbance is measured at a wavelength of 405 nm, and CAT activity is calculated using a standard curve.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III

Key Parameters

• Assay Method: Colorimetric method (Ammonium Molybdate Chromogenic Method)
• Detection Range: 3-195 mM
• Sensitivity: 3 mM
• Sample Types: Animal and plant tissues, cells/bacteria, serum (plasma)

Technical Advantages

• Clear operating procedure, no specialized instrumentation required
• Stable chromogenic development with good reproducibility
• Suitable for high-throughput sample analysis

Precautions

• A preliminary experiment is recommended to optimize sample amount
• Sample protein concentration normalization using the BCA method is recommended
• Users must establish their own standard curve
• Operations should be performed in a fume hood with protective equipment such as gloves

Storage and Shipping

• Storage Conditions: Store protected from light at 4°C, stable for 6 months
• Shipping Method: Shipped on blue ice packs

Safety Warning

The biochemical reagents contained in this product possess certain irritancy and toxicity. Strictly adhere to laboratory biosafety protocols during operation.

Application Background

Catalase (CAT) is a key enzyme in the cellular antioxidant system, responsible for catalyzing the decomposition of H₂O₂ to prevent cellular damage caused by accumulation of reactive oxygen species. It holds significant value in research on mechanisms of oxidative stress-related diseases.

Specifications and Pricing

Search Aliases: Catalase, CAT, Catalase Activity Assay Kit

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yacoin Biotechnology for specific details.