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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃,避光保存 6 个月
- 保质期:
4℃,避光保存 6 个月
- 英文名:
Micro Asparagine Synthetase (AS) Activity Assay Kit
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
48 T/48 S/96 T/96 S
| 规格: | 48 T/48 S | 产品价格: | ¥669.0 |
|---|---|---|---|
| 规格: | 96 T/96 S | 产品价格: | ¥1098.0 |
CheKine™ 天冬酰胺合成酶(AS)活性检测试剂盒(微量法)
货号:KTB3046-E
英文全称:Micro Asparagine Synthetase (AS) Activity Assay Kit
产品概述
CheKine™ 天冬酰胺合成酶(AS)活性检测试剂盒(微量法)是一款基于比色法原理开发的生化检测试剂盒,专为精准定量各类生物样本(包括动植物组织、细菌、细胞、血清/浆等)中的天冬酰胺合成酶(AS)活性而设计。AS是广泛存在于生物体内的一种关键氨基转移酶,其在氨解毒代谢及氨基酸合成途径中扮演重要角色。
检测原理
本试剂盒通过检测AS催化L-天冬酰胺水解生成L-天冬氨酸和氨的反应速率来定量酶活性。反应产生的氨与奈氏试剂发生显色反应,通过测定特定波长下的吸光度值变化,即可准确计算出AS的活性水平。
试剂盒组分
- Reagent I
- Reagent Ⅱ
- Reagent Ⅲ
- Reagent Ⅳ
- Reagent Ⅴ
- Reagent VI
- Standard
产品规格与价格
| 规格 | 价格(元) | 货期 |
|---|---|---|
| 48 T/48 S | 669 | 现货(次日发货) |
| 96 T/96 S | 1098 | 现货(次日发货) |
样本处理与保存建议
• 推荐使用新鲜样本进行检测。若需保存,请于-80℃条件下冻存,可维持数周稳定性。
• 实验前解冻样本时,需严格控制解冻温度与时间。如在室温下解冻,请在4小时内完成全部操作,以避免酶活性降解。
保存与运输
保存条件:4℃避光保存,有效期6个月。
运输条件:采用蓝冰冰袋低温运输,以确保试剂稳定性。
注意事项
实验前请确保所有试剂组分及实验设备均预热至适宜的操作温度,以保证检测结果的准确性与重复性。
警告:本产品仅限用于科学研究用途,不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定,本公司对因使用本产品可能导致的专利侵权或其他违规行为不承担任何责任。
别名:天冬酰胺合成酶(AS)活性检测试剂盒,AS Activity Assay Kit
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

CheKine™ Micro Asparagine Synthetase (AS) Activity Assay Kit
Catalog Number: KTB3046-E
Full Name: Micro Asparagine Synthetase (AS) Activity Assay Kit
Overview
The CheKine™ Micro Asparagine Synthetase (AS) Activity Assay Kit is a biochemically optimized colorimetric assay kit designed for the precise quantification of asparagine synthetase (AS) activity in a variety of biological samples, including animal and plant tissues, bacteria, cells, serum/plasma, etc. AS is a key aminotransferase widely present in living organisms, playing a vital role in ammonia detoxification metabolism and amino acid biosynthesis pathways.
Assay Principle
This kit quantifies AS activity by measuring the reaction rate at which AS catalyzes the hydrolysis of L-asparagine to produce L-aspartic acid and ammonia. The ammonia produced reacts with Nessler's reagent to generate a colorimetric signal. The change in absorbance at a specific wavelength is measured to accurately calculate the AS activity level.
Kit Components
- Reagent I
- Reagent Ⅱ
- Reagent Ⅲ
- Reagent Ⅳ
- Reagent Ⅴ
- Reagent VI
- Standard
Specifications and Pricing
| Specification | Price (RMB) | Availability |
|---|---|---|
| 48 T/48 S | 669 | In stock (shipped next day) |
| 96 T/96 S | 1098 | In stock (shipped next day) |
Sample Handling and Storage Recommendations
• Fresh samples are recommended for assay. If storage is necessary, freeze at -80°C to maintain stability for several weeks.
• Thaw samples under controlled temperature and time conditions prior to the experiment. If thawing at room temperature, complete all procedures within 4 hours to prevent enzyme activity degradation.
Storage and Shipping
Storage Conditions: Store protected from light at 4°C; valid for 6 months.
Shipping Conditions: Shipped with blue ice packs to ensure reagent stability.
Precautions
Prior to the experiment, ensure all kit components and equipment are pre-equilibrated to the appropriate operating temperature to guarantee the accuracy and reproducibility of the results.
Warning: This product is for research use only. Not intended for human or clinical diagnostic use. The user is responsible for ensuring that their application does not infringe any patents or licensing agreements. The company assumes no liability for patent infringement or other violations resulting from the use of this product.
Also Known As: Asparagine Synthetase (AS) Activity Assay Kit, AS Activity Assay Kit
Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult with YaKeyin Company.
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