GLS试剂盒，用于样本中谷氨酰胺酶活性检测，GLS Acti

CheKine™ 微量谷氨酰胺酶（GLS）活性检测试剂盒

货号：KTB3043-E

产品类型：细胞代谢检测试剂盒（比色法）

产品概述

CheKine™ Micro Glutaminase (GLS) Activity Assay Kit 是一款用于精准定量多种生物样本中谷氨酰胺酶（GLS）活性的高特异性检测工具。该试剂盒采用微量比色法原理，适用于动植物组织、细菌、细胞裂解液以及血清/血浆等多种样本类型，助力研究人员深入探究细胞代谢过程中氮素代谢与氨调控机制。

检测原理

谷氨酰胺酶（GLS）催化谷氨酰胺水解生成L-谷氨酸与氨。本试剂盒通过奈氏试剂（Nessler's Reagent）显色反应检测反应体系中氨的生成速率，进而精确计算GLS酶活性，方法稳定可靠，灵敏度高。

试剂盒组分

• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
• Reagent VI
• Standard

样本处理建议

推荐使用新鲜样本进行检测。若需保存，请置于-80℃环境，并可稳定数周。检测前应在室温环境下解冻，并确保在4小时内完成样品准备与检测，以维持酶活性的真实性。

保存与运输

试剂盒应在4℃避光条件下保存，有效期为6个月。所有产品均采用蓝冰冰袋运输，以保证试剂稳定性。

应用领域

• 细胞代谢研究
• 氮代谢通路分析
• 肿瘤细胞能量代谢研究
• 细菌与植物根中氨代谢机制探讨

规格与价格

规格	价格（元）	货期
48 T/48 S	1089	现货（次日发货）
96 T/96 S	1798	现货（次日发货）

注意事项

实验前请确保所有试剂及设备处于适宜温度。本产品仅限研究使用，不用于人类或临床诊断。使用前请阅读相关文献并确认符合当地法规与知识产权要求。

别名：谷氨酰胺酶（GLS）检测试剂盒 | GLS Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Glutaminase (GLS) Activity Assay Kit

Catalog Number: KTB3043-E

Product Type: Cell Metabolism Assay Kit (Colorimetric Method)

Product Overview

The CheKine™ Micro Glutaminase (GLS) Activity Assay Kit is a highly specific tool designed for the accurate quantification of glutaminase (GLS) activity in various biological samples. Utilizing a micro-colorimetric principle, this kit is suitable for a wide range of sample types, including animal and plant tissues, bacteria, cell lysates, and serum/plasma, aiding researchers in the in-depth investigation of nitrogen metabolism and ammonia regulatory mechanisms in cellular metabolic processes.

Assay Principle

Glutaminase (GLS) catalyzes the hydrolysis of glutamine to produce L-glutamate and ammonia. This kit employs Nessler's Reagent-based colorimetric detection to measure the rate of ammonia generation in the reaction system, thereby precisely calculating GLS enzyme activity. The method is stable, reliable, and highly sensitive.

Kit Components

• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
• Reagent VI
• Standard

Sample Handling Recommendations

Fresh samples are recommended for assay. If storage is necessary, samples should be kept at -80°C and remain stable for several weeks. Prior to assay, thaw samples at room temperature and ensure that sample preparation and testing are completed within 4 hours to maintain the authenticity of enzyme activity.

Storage and Shipping

The kit should be stored protected from light at 4°C and is stable for 6 months. All products are shipped with blue ice packs to ensure reagent stability.

Applications

• Research on Cellular Metabolism
• Analysis of Nitrogen Metabolic Pathways
• Studies on Energy Metabolism in Tumor Cells
• Investigation of Ammonia Metabolism Mechanisms in Bacteria and Plant Roots

Specifications and Pricing

Specification	Price (¥)	Lead Time
48 T/48 S	1089	In Stock (Ships Next Day)
96 T/96 S	1798	In Stock (Ships Next Day)

Precautions

Ensure all reagents and equipment are at appropriate temperatures before starting the experiment. This product is for research use only, not for human or clinical diagnostic use. Please review relevant literature and confirm compliance with local regulations and intellectual property requirements prior to use.

Also Known As: Glutaminase (GLS) Assay Kit | GLS Activity Assay Kit

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult YaKeYin Company.