胰蛋白酶活性检测试剂盒，用于对样本中TRYP活性检测，Try

CheKine™ 胰蛋白酶活性检测试剂盒（微量法）

货号：KTB2320-C

产品类型：细胞代谢检测试剂盒

检测指标：Trypsase

样本类型：适用于动植物组织样本

产品概述

CheKine™ 胰蛋白酶活性检测试剂盒（微量法）是一款用于精确测定动植物组织样本中胰蛋白酶（Trypsase）活性的核心产品。该试剂盒基于酶促反应原理，通过检测555 nm波长处吸光值的降低速率，实现对胰蛋白酶活性的高效、稳定量化。

检测原理

胰蛋白酶可选择性水解TAME的酯键，释放出游离羧基并与氢`氧`化`钠发生中和反应，引起反应体系pH值下降。以苯`酚红作为指示剂，在555 nm处监测吸光度变化，酶活性在一定浓度范围内与吸光度降低值呈良好线性关系，确保检测结果准确可靠。

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ

特点与优势

• 专为动植物组织样本优化，具备高特异性与灵敏度；
• 提供详尽的样本制备指南与活性计算公式，便于用户快速开展实验与数据分析；
• 试剂稳定性强，批间差小，支持重复性实验需求。

适用场景

本产品适用于细胞代谢研究、消化酶功能分析、病理状态下的酶活性变化监测等科研领域。

保存与运输

保存条件：4℃避光保存，有效期6个月。

运输方式：蓝冰冰袋运输，确保试剂稳定性。

注意事项

• 建议使用新鲜样本，若需保存，请于-80℃环境下存放，避免反复冻融；
• 解冻样本时需严格控制温度与时间，室温解冻应在4小时内完成；
• 实验前请确保所有试剂及设备处于适宜温度，以保证反应体系稳定性。

规格与价格

规格	价格（元）	货期
48 T/48 S	598	现货（次日发货）
96 T/96 S	998	现货（次日发货）

警告：本产品仅用于科学研究，不适用于人类或临床诊断。用户应确保使用行为不违反相关专利或许可协议，本公司不对因使用本产品导致的专利侵权或其他违规行为承担责任。

别名：胰蛋白酶；胰酶；胰蛋白酶活性检测试剂盒（Trypsase Activity Assay Kit）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Trypsase Activity Assay Kit (Micro Scale)

Catalog Number: KTB2320-C

Product Type: Cell Metabolism Assay Kit

Assay Target: Trypsase

Sample Type: Suitable for plant and animal tissue samples

Product Overview

The CheKine™ Trypsase Activity Assay Kit (Micro Scale) is a core product designed for the precise determination of trypsase activity in plant and animal tissue samples. Based on the enzymatic reaction principle, this kit enables efficient and stable quantification of trypsase activity by monitoring the rate of decrease in absorbance at 555 nm.

Assay Principle

Trypsase selectively hydrolyzes the ester bond of TAME, releasing free carboxyl groups that undergo a neutralization reaction with sodium hydroxide, resulting in a decrease in the pH of the reaction system. Using phenol red as an indicator, the change in absorbance is monitored at 555 nm. Enzyme activity exhibits a good linear relationship with the decrease in absorbance within a certain concentration range, ensuring accurate and reliable results.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III

Features and Advantages

• Optimized specifically for plant and animal tissue samples, offering high specificity and sensitivity;
• Comprehensive sample preparation guidelines and activity calculation formulas are provided to facilitate rapid experimentation and data analysis;
• Strong reagent stability with minimal batch-to-batch variation, supporting reproducible experimental requirements.

Applications

This product is suitable for research areas such as cell metabolism studies, digestive enzyme function analysis, and monitoring enzyme activity changes under pathological conditions.

Storage and Shipping

Storage Conditions: Store protected from light at 4°C; valid for 6 months.

Shipping Method: Shipped with blue ice packs to ensure reagent stability.

Precautions

• Fresh samples are recommended. If storage is necessary, samples should be kept at -80°C and repeated freeze-thaw cycles should be avoided;
• Strictly control temperature and time during sample thawing; thawing at room temperature should be completed within 4 hours;
• Ensure all reagents and equipment are at appropriate temperatures prior to the experiment to maintain reaction system stability.

Specifications and Pricing

Specification	Price (RMB)	Availability
48 T/48 S	598	In stock (shipped next day)
96 T/96 S	998	In stock (shipped next day)

Warning: This product is intended for research use only and is not suitable for human or clinical diagnostic use. Users should ensure that their use does not violate any relevant patents or licensing agreements. The company is not responsible for any patent infringement or other violations resulting from the use of this product.

Alias: Trypsin; Pancreatin; Trypsase Activity Assay Kit

Note: The above information may be subject to change due to product optimization and upgrades. For specific details, please consult with Yakeyin Company.