Acetyl-CoA试剂盒，用于样本中乙酰辅酶A含量检测，A

Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit

产品概述

Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit（货号：KTB1260-E）是一款基于比色法原理开发的细胞代谢检测试剂盒，专为精确检测多种生物样本（包括动植物组织、微生物及培养细胞）中的乙酰辅酶A含量而设计。该试剂盒适用于三羧酸循环相关研究，为核心代谢通路分析提供可靠工具。

检测原理

试剂盒通过酶促反应将乙酰辅酶A转化为可测量产物，利用NADH在340 nm处的吸光度变化进行定量分析，具备高灵敏度和特异性，检测范围达1.5–3200 nmol/mL。

产品特点

• 操作简便：优化的比色法流程，步骤清晰，缩短实验时间。
• 广泛样本兼容性：适用于动物组织、植物样本、微生物及培养细胞等多种样本类型。
• 详细指南支持：提供全面的样品制备方案与结果计算方法，降低操作差异。
• 高灵敏度与线性范围：可准确检测低至纳摩尔级别的乙酰辅酶A浓度。

试剂盒组分

• Extraction Buffer
• Reagent Ⅰ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Standard (NADH)

应用背景

乙酰辅酶A（Acetyl-CoA）作为能量代谢中的核心中间产物，广泛参与三羧酸循环、脂肪酸合成、酮体生成及胆固醇生物合成等关键生化过程。本试剂盒为研究细胞代谢调控、能量平衡和代谢疾病机制提供了有效的分析工具。

规格与价格

规格	价格（元）	货期
48 T/48 S	3416	现货（次日发货）
96 T/96 S	5689	现货（次日发货）

保存与运输

产品需避光保存于-20℃。运输条件为冰袋（蓝冰）低温保护，以确保试剂稳定性。自收货日起，在推荐保存条件下有效期为6个月。

注意事项

• 请勿混用不同批次或厂家的组分，以免影响实验结果。
• 操作时避免产生气泡，勤换吸头，防止交叉污染。
• 实验前确保所有试剂及设备处于适宜温度，样本提取后请置于冰上以防降解。
• 建议使用新鲜样本进行提取，以保持酶活力。
• 如吸光度值异常，可调整样本量或Extraction Buffer体积进行优化。

警告

本产品仅用于科研用途，不适用于人类或临床诊断。使用者应确保应用方式不侵犯任何专利或许可协议，厂家不对因使用本产品导致的专利侵权或其他违规行为承担责任。

如需进一步了解技术细节或实验支持，欢迎联系我们的技术支持团队。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit

Product Overview

The Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit (Catalog Number: KTB1260-E) is a cell metabolism detection kit developed based on colorimetric principles, specifically designed for the precise quantification of acetyl-CoA levels in various biological samples, including animal and plant tissues, microorganisms, and cultured cells. This kit is suitable for tricarboxylic acid (TCA) cycle-related research, providing a reliable tool for core metabolic pathway analysis.

Assay Principle

The kit utilizes an enzymatic reaction to convert acetyl-CoA into a measurable product, quantifying the change in absorbance of NADH at 340 nm. It offers high sensitivity and specificity, with a detection range of 1.5–3200 nmol/mL.

Features

• Easy Operation: An optimized colorimetric procedure with clear steps reduces experimental time.
• Broad Sample Compatibility: Suitable for various sample types, including animal tissues, plant samples, microorganisms, and cultured cells.
• Comprehensive Guidance: Provides detailed sample preparation protocols and result calculation methods to minimize operational variability.
• High Sensitivity and Linear Range: Accurately detects acetyl-CoA concentrations as low as nanomolar levels.

Kit Components

• Extraction Buffer
• Reagent Ⅰ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Standard (NADH)

Background

Acetyl-CoA, a central intermediate in energy metabolism, is extensively involved in key biochemical processes such as the tricarboxylic acid cycle, fatty acid synthesis, ketogenesis, and cholesterol biosynthesis. This kit serves as an effective analytical tool for studying cellular metabolic regulation, energy balance, and mechanisms of metabolic diseases.

Specifications and Pricing

Specification	Price (¥)	Delivery Time
48 T/48 S	3416	In Stock (Ships Next Day)
96 T/96 S	5689	In Stock (Ships Next Day)

Storage and Shipping

The product should be stored protected from light at -20°C. It is shipped with ice packs (blue ice) to ensure reagent stability. The shelf life is 6 months from the date of receipt when stored under recommended conditions.

Precautions

• Do not mix components from different batches or manufacturers to avoid affecting experimental results.
• Avoid introducing bubbles during operation; change pipette tips frequently to prevent cross-contamination.
• Ensure all reagents and equipment are at appropriate temperatures before starting the experiment. Place extracted samples on ice to prevent degradation.
• Fresh samples are recommended for extraction to maintain enzyme activity.
• If absorbance values are abnormal, adjust sample volume or Extraction Buffer volume for optimization.

Warning

This product is for research use only and is not intended for human or clinical diagnostic use. Users should ensure that their application does not infringe any patents or licensing agreements. The manufacturer is not liable for any patent infringements or other violations resulting from the use of this product.

For further technical details or experimental support, please contact our technical support team.

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Yakeyin Company.