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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
Polyclonal
- 抗体名:
辣根过氧化物酶标记的干扰素诱导蛋白P78抗体
- 宿主:
Rabbit
- 形态:
Lyophilized or Liquid
- 应用范围:
ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20°C
- 规格:
100ug
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
Wholemount staining of embryos
as above 15 to 30''. Secondary antibody is added in blocking solution. Concentration varies, but 1/500 works for most. For monoclonal primaries use anti-mouse coupled to AP or HRP, for polyclonals use anti-rabbit coupled AP or HRP. Incubate at 4 degC
: Indirect immunohistochemistry with avidin-biotin peroxidase(e.g rabbit anti antigen X, goat anti-rabbit biotin, avidin HRP) 1- Wash twice in TBS 0.05M pH7.5, to which 0.01% Tween 20 has been added. 2- Briefly blot the slides without letting
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